Dissertation
Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology

Effect of HuaZhuo Jiedu TiaoGan Decoction on the MMP-1 and TIMP-1 in Rats Model of Immunity Hepatic Fibrosis

Author YuQin
Tutor LiDianGui
School Hebei Medical University
Course Traditional Chinese Medicine
Keywords ZhuoDu theory HuaZhuo JieDu TiaoGan decoction Hepatic fibrosis Matrix-metalloproteinase Tissue inhibitor of metalloproteinase
CLC R285.5
Type Master's thesis
Year 2010
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Objectives:The research based on the theory of ZhuoDu. By observing the effect of HuaZhuo JieDu TiaoGan decoction on pathological liver tissue ,MMP-1 and TIMP-1 in rats model of immunity hepatic fibrosis,to discuss the mechanism of antifibrotic action further.Methods:60 SPF level weighed 200-220 grams Wistar rats randomly divided into six groups:the normal group, the model group, HuaZhuo JieDu TiaoGan decoction high dose group(HJT high dose group for short), HuaZhuo JieDu TiaoGan decoction middle dose group(HJT middle dose group) , HuaZhuo JieDu TiaoGan decoction low dose group(HJT low dose group) , colchicine treatment group(western medicine treatment group) ,with 10 rats for each group .Except the normal group, established pig’s serum( 0.5ml per mouse, twice a week) in rats by intraperitoneal injection for eight weeks. The treatment groups were given drug at the end of the eighth week. The equal saline(2ml each time) was given to the normal control group and the model group. Each group executes separately in the treatment 4th weekend. The livers were taken and the degree of hepatic fibrosis was judged by routine haematoxylin-eosin staining. MMP-1,TIMP-1 expression were determined using immunohistochemical technique .Data was collected and analyzed through test or analysis of variance. The statistic software was SPSS13.0.Results:1 Effect of HuaZhuo JieDu TiaoGan decoction on the levels of body weight, liver weight, spleen weight in rats model of immunity hepatic fibrosis. Compared with the normal control group and the model group, the body weight of treatment groups decreased significantly (P<0.05).The liver weight, spleen weight increased significantly (all P<0.05); Compared with the normal control group,the body weight, liver weight and spleen weight of the TCM treatmengt groups, there is no significant difference (all P> 0.05). Compared with the model group, the body weight of TCM treatmengt groups increased significantly (P <0.05), liver weight and spleen weight reduced significantly (P <0.05).The body weight of TCM high dose group is higher than western medicine treatment group (P <0.05). The dose of TCM treatmengt groups showed no significant difference (P> 0.05).2 Effect of HuaZhuo JieDu TiaoGan decoction on the levels of pathomorphology in rats model of immunity hepatic fibrosis.The structures of hepatic lobule are integrated, hepatic cord neatly ranged in the center of navel vein.There is not degeneration and necrosis in the liver tissue. The model group liver organization pathological change region are many, the area is big, the normal hepatic lobe disarrangement of the structure, collects the district expansion to have the massive inflammatory cell infiltration, the liver cell swelling, the arrangement is crowded, sees the size different vacuole, the fatty degeneration to be widespread, has a part of necrosis, collects around the district and the central vein the Fiber structure proliferation. Western medicine group obvious hepatic lobe structure basic normal, the liver cell swelling denaturation is obvious by the central vein around, the peripheral region is light, the phlogocyte infiltration, the Fiber structure proliferation compare the model group to reduce obviously. Big medicinal preparation group obvious hepatic lobe structure basic normal, the liver organization pathological change region are few, the extent of damage is light, the liver cell arrangement is normal, only sees around the central vein the liver cell vacuole type denaturation. The medicinal preparation group pathological change region area small medicinal preparation group is small. The small medicinal preparation group liver cell inflammation is not remarkable, obviously few fatty degeneration vacuole, hepatic lobe structure basic normal, the liver cell arrangement was still neat, around the central vein the visible part liver cell denaturates, the pathological change region area compares the model group, the western medicine group to be small, and the liver cell denatured degree is light. 3 Experimental study on the effect of HuaZhuo JieDu TiaoGan decoction on MMP- 1 in rats model of immunity hepatic fibrosis.MMP-1 expressed mainly in the cells between the fiber spacing and periportal mesenchymal, stained brown. Intensity of its expression followed as: HJT high dose group , HJT middle dose group, HJT low dose group, western medicine group, model group. The expression in the normal control group is weakest.There are significant difference between the model group and the traetment groups(P<0.05).Indicate that the treatment group can activate the MMP-1 expression; There is significant difference between western medication group and normal group(P<0.05); There is significant difference between HJT high dose group and western medicine group (P<0.05); There is no significant difference between HJT high dose group, HJT middle dose group and HJT low dose group(P> 0.05) .That is to say,the TCM treatment groups show a better effect on the expression of the MMP-1.4 Experimental study on the effect of HuaZhuo JieDu TiaoGan decoction on TIMP- 1 in rats model of immunity hepatic fibrosis.Intensity of TIMP- 1 expression followed as: model group , western medicine group ,HJT high dose group , HJT middle dose group, HJT low dose group. The expression in the normal control group is weakest. A little TIMP-1 positive cells are visible in the normal rat liver tissue. In the model group the The proportion of positive cells in rat liver tissues were significantly higher than the the normal group . uneven brown granular material are visible. Compared with the model group, TIMP-1 protein expression in the TCM treatment groups decreased significantly (P<0.05); There is significant difference between western medication group and normal group(P<0.05); There is significant difference between HJT high dose group and western medicine group (P<0.05); There is no significant difference between HJT high dose group, HJT middle dose group and HJT low dose group(P> 0.05). Therefore, the TCM treatment groups show a better effect on the expression of the TIMP-1.Conclusions: 1 HuaZhuo JieDu TiaoGan decoction can improve the general state of rats model of immunity hepatic fibrosis.2 HE pigmentation result show: HuaZhuo JieDu TiaoGan decoction can recover the texture fibrous of hepatic fibrosis,and can raise the ability of fibrous solution.3 HuaZhuo JieDu TiaoGan decoction can improve the content of MMP-l,and drepress obviously of TIMP-1, which might play a role in regulating the disequilibrium between MMPS and TIMPS, promoting the degrading of ECM, decreaing the deposition of ECM,so it can resolve fibrous of liver.4 The result shows that the clinicao value of HuaZhuo JieDu TiaoGan decoction is very high and it make a new gateway to the therapy of Hepatic fibrosis.

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