Dissertation
Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology

Rhizoma Coptidis Inhibits LPS-induced MCP-1 Production in Murine Macrophages

Author WangHongJie
Tutor ZengHeSong;Blessing Erwin
School Huazhong University of Science and Technology
Course Internal Medicine
Keywords Coptis Berberine Transcription factor MCP-1 Inflammation Macrophages
CLC R285
Type Master's thesis
Year 2011
Downloads 22
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Purpose. Research Coptis extract on LPS-induced macrophage inflammatory response and its molecular mechanism. 2. Explore Coptis extract the main effect of the composition and with the commonly used anti-inflammatory drug efficacy comparison. Method 1. Different concentrations Coptis extract LPS-induced macrophages incubated for different times to collect nuclear protein, using the the EMSA method of detection of inflammation-related nuclear transcription factors (AP-1 and NFκB) activity, cell supernatant was detected by ELISA The MCP-1 concentrations. 2 different concentrations of of berberine and commonly used anti-inflammatory drugs (statins, angiotensin converting enzyme inhibitors (ACEI) or angiotensin receptor blockers (ARB)) and LPS-induced macrophages were incubated for different times to collect nuclear protein, using the the EMSA method of detection of inflammation-related nuclear transcription factors (AP-1 and NFκB) activity, and MCP-1 concentrations in cell supernatants was measured by ELISA. Results. Compared with the control group, berberine on RAW 264.7 morphological no significant impact. 2. Coptis extract can inhibit LPS-stimulated RAW 264.7 cell transcription factor AP-1 and NFκB activity in a time-and dose-dependent, but reduced activity of NFκB longer incubation time and higher concentration of the extract. Coptis extract alkaloid ingredient berberine can inhibit LPS-stimulated RAW 264.7 cell transcription factor AP-1 and NFκB activity. Reductase Inhibitors (Statins) in HMGCo-A and angiotensin converting enzyme inhibitors (ACEI) or angiotensin receptor inhibitors ARB) and other drugs, the only ARB olmesartan small increments, but significant places inhibition of LPS-induced RAW 264.7 cell transcription factor AP-1 activity, and other drugs (rosuvastatin statin, fluvastatin telmisartan) AP-1 activity of no significant impact. All of the above comparison drug activity of NFκB no significant impact. 5 Coptis extract and berberine could significantly inhibit the secretion of MCP-1 in LPS-stimulated RAW 264.7 cells in a time-and dose-dependent manner. However, other anti-inflammatory drugs, statins and angiotensin-converting enzyme inhibitors (ACEI) or angiotensin receptor blockers (ARB) has no effect on the secretion of MCP-1 in LPS-stimulated RAW 264.7 cells. Compared with the result of the detection of the activity of transcription factor, a low concentration of berberine extract or berberine can be significantly suppressed RAW 264.7 cells of MCP-1 secretion. Conclusion 1. Coptis extract can effectively reduce the LPS-induced RAW 264.7 cell inflammatory response. 2. Berberine Coptis extract play the main component of the anti-inflammatory efficacy and its strong anti-inflammatory Statins, ACEIs and ARBs. 3 Anti-inflammatory effect of the berberine is at least in part achieved through the inhibition of MCP-1 production, suppressing cell-factor (MCP-1) the secretion efficacy of not just dependent pathway mediated by the nuclear transcription factor AP-1 and NFκB.

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