Clinical Significance of Hepatitis B Virus Core Gene PCR-RFLP Patterns on the Course of Hepatocellular Carcinoma and Liver Cirrhosis in China.
|School||Huazhong University of Science and Technology|
|Course||Clinical Laboratory Division|
|Keywords||Hepatitis B PCR-RFLP pattern Hepatocellular carcinoma Cirrhosis|
Introduction : Hepatitis B is a disease caused by the hepatitis B virus . It is spread through infected blood and body fluids . There are around 400 million people infected with the hepatitis B virus , and about 1.5 million deaths a year . In China alone there are 130 million people infected with the hepatitis B virus . China each year about 30 million deaths , including 180,000 patients with hepatocellular carcinoma . HBV viral DNA contains four open reading frames : the core , the surface , x and polymerase . Core gene encoding hepatitis B e antigen and hepatitis B core antigen . The hepatitis B virus core gene mutation and sNPs of patients with chronic hepatitis B hepatitis activity . Goals : 1 probe cleared hepatocellular carcinoma and cirrhosis in patients with hepatitis B virus core region PcR . RFLP pattern . 2 Evaluation of PCR-RFLP pattern of clinical significance in patients with hepatocellular carcinoma and cirrhosis . MATERIALS AND METHODS: collected from January 1, 2010 to November 2010 , a total of 320 patients with hepatocellular carcinoma and 130 patients with cirrhosis . Based on uniform criteria to select patients . The patient's age range from 14 years old to 82 years old . Infected with the AIDS virus, hepatitis C virus , or thyroid disease were excluded . A -PCR is used for amplification of HBV-DNA . For hepatitis B virus , hepatocellular carcinoma and cirrhosis of the experimental analysis of the molecular biology research : HBV DNA , PCR-RFLP pattern sequencing results : the five typical PcR-RFLP restriction enzyme cleavage _rsp509I mode (c, D, E , G c / G) was found . RFLP pattern of distribution , E - 8.7% , D 2.5% CC- 62.8 % , G - 13.7% and C / G mixture-12.2% was found in the 320 cases of hepatocellular carcinoma ; C-57%, E-11.5% G-17%, D-4.6%, and C/Gmixture-10% at 130 cirrhosis cases is found. In both cases , it is clear that the RFLP pattern D is the lowest. PCR-RFLP pattern distribution in no significant difference between the HCC and cirrhosis . Conclusion: RFLP mode C patients with serum AFP. HBV-DNA, serum ALT was significantly higher than the other modes . Despite HBv DNA at a high level , but PcR RFLP C / G mode patients have low levels of serum AFP and ALT .