The Plasma and Tissue Expression of Osteopontin and CD44v6 in Gastric Carcinoma and Their Significance
|School||Huazhong University of Science and Technology|
|Keywords||Gastric cancer Osteopontin CD44V6 Serum Immunohistochemistry|
Objective: To detect osteopontin (OPN) and CD44v6 levels in the serum of patients with gastric cancer and gastric cancer tissues expression, to analyze the relationship between the expression of both the clinical and pathological features of gastric cancer, further elaborated the development of OPN and CD44v6 in gastric The role. Methods: From February 2010 to July 2010 in our hospital gastrointestinal surgery gastric cancer patients with pathologically confirmed 40 cases, including 27 males and 13 females, aged 24-76 years, mean age 52 years, preoperative no treatment. The control group of 20 normal serum from our hospital in normal healthy subjects, gastric mucosa specimens were obtained from 20 parts paraneoplastic more than 5cm from the cancer tissue edge. Double antibody sandwich - ELISA serum of 40 patients with gastric cancer, 20 normal healthy serum OPN and CD44v6 content detected by immunohistochemistry SP method 40 gastric carcinoma after surgical resection specimens and 20 copies paraneoplastic expression of OPN and CD44v6 in gastric mucosa specimens. Results: 1. Gastric cancer patients with preoperative serum OPN (105.54 ± 62.52) ng / ml was significantly higher than normal healthy subjects (P = 0.0087), cancer tissue expression of OPN expression was significantly higher than in the gastric mucosa paracancerous ( 75% vs 10%, P value 0.0021), the expression of OPN in serum and tissues with the histological grade, increased clinical stage and lymph node metastasis and elevated, was a positive correlation. Gastric cancer patients with preoperative serum the CD44v6 (177.21 ± 46.48) ng / ml is higher than normal healthy subjects (P = 0.0055), cancer tissue positive expression rate of CD44v6 in gastric mucosa (77.5% vs 15 paracancerous %, P = 0.0042), the expression of CD44v6 in serum and tissues with the histological grade, increased clinical stage and lymph node metastasis increased and was positively correlated. Comparing 40 patients with gastric cancer before surgery correlation between serum OPN content and CD44v6 content, r = 0.038, P lt; 0.05, show a positive correlation. Conclusion: 1. Gastric cancer patients with preoperative serum and tissue OPN expression was significantly higher, with the histological grade, clinical stage rises higher and lymph node metastasis was positively correlated. That OPN is involved in gastric carcinogenesis may reflect body tumor load status and predict tumor progression. Gastric cancer patients with preoperative serum and tissue CD44v6 expression was significantly higher with the histological grade, increased clinical stage and lymph node metastasis increased and was positively correlated. Suggesting that CD44v6 may promote the formation of gastric expression changes in the level of CD44v6 in gastric cancer serum and tissue can be used as biological indicators of gastric cancer progression dynamic observation. Gastric cancer patients with preoperative serum There was a positive correlation between OPN content and CD44v6 content that OPN and CD44v6 incidence of gastric cancer development may have a synergistic effect, the combined detection of both serum levels may provide new biological diagnosis of gastric cancer school basis.