Dissertation
Dissertation > Agricultural Sciences > Aquaculture, fisheries > Aquaculture technology > Aquatic animal feed nutrition > Compound feed

Study on Utilization of Cottonseed Meal and Canola Meal for the Pacific White Shrimp, Litopenaeus Vannamei

Author WangTuo
Tutor ZhouQiCun
School Guangdong Ocean University
Course Aquaculture
Keywords Litopenaeus vannamei Cottonseed meal Canola Grow Apparent digestibility Non-specific immune
CLC S963.7
Type Master's thesis
Year 2010
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The complex of cottonseed meal, rapeseed meal, cottonseed meal and rapeseed meal replacement of fish meal Litopenaeus vannamei growth, feed utilization, body composition, and non-specific immune parameters of experimental results are as follows: 1. Study cottonseed meal alternative fishmeal Litopenaeus vannamei growth, feed utilization, the 8-week growth experiment. Using cottonseed meal instead of 8%, 16%, 24%, 32%, 40% fish meal, with the addition of 30% of the fish meal in feed (crude protein 44%) for the control group, dubbed the six kinds of nitrogen can feed, the the Litopenaeus vannamei initial weight of 0.80 ± 0.01g. The experimental results show that the feed cottonseed meal Litopenaeus vannamei survival and liver body than no significant impact, as cottonseed meal usage increases, the growth of juvenile shrimp decreased after increasing trend. 8% alternative set of final body weight, weight gain, specific growth rate was the highest, lowest feed conversion ratio, no significant difference between groups. When the feed cottonseed meal replacement reached 24%, and its protein efficiency significantly less than 8% of the alternative group. The Litopenaeus vannamei digestibility data analysis showed that 8% cottonseed meal replacement group protein, crude fat apparent digestibility slightly higher than that in the control group, significantly higher than other groups; body composition analysis results show that, cottonseed meal replacement level from 24% to 40%, a phosphorus content of the whole shrimp and muscle is significantly reduced. Growth, apparent digestibility indicators feed cottonseed meal instead of 8% fish meal (ie cottonseed meal in feed added amount of 3.4%) in favor of growth. Growth as an indicator, cottonseed meal, fish meal replacement ratio is not higher than 24% (i.e., the addition amount of the cottonseed meal of not more than 11%) suitable. Research canola replace fish meal Litopenaeus vannamei growth, feed utilization, the 8-week growth experiment. Canola meal instead of 8%, respectively, 16%, 24%, 32%, 40% of fish meal to feed the addition of 30% of the fish meal (crude protein 42%) for the control group, dubbed the six kinds of nitrogen can feed, and the the Litopenaeus vannamei initial weight of 0.62 ± 0.01g. Experimental results show that canola meal replacement of fish meal ratio does not exceed 32% in the feed, Litopenaeus vannamei rate of weight gain, specific growth rate, feed conversion ratio and the control group, compared with no significant difference in average weight tail, specific growth rate protein efficiency in 8% double low rapeseed meal replacement group the highest. Add canola meal feed of Litopenaeus vannamei survival rate no significant impact. On the Litopenaeus vannamei digestibility data analysis showed that the dry matter, protein and crude fat apparent digestibility of the control group was significantly higher than canola add a group, the canola alternative group between dry matter apparent The Digestibility no difference. 8% substitution group apparent digestibility of protein and crude fat were higher than other canola meal replacement group and significantly higher than the 40% replacement group. Body composition analysis showed that 32% and 40% canola meal replacement group shrimp ash significantly higher hepatopancreas crude fat was significantly lower. The groups feed soaked in water weight loss rate and canola meal replacement level positively correlated. Growth as an indicator, the proportion of canola meal replacement of fish meal feed is not higher than 24% (ie, the added amount of double-low rapeseed meal in the feed to no more than 12%) is appropriate. 3 for the study of cottonseed meal, double low rapeseed meal complex alternative to fishmeal and adding crystalline amino acids on the growth of Litopenaeus vannamei feed utilization, as well as the impact of the non-specific immune indicators, eight-week growth experiment. The added amount of 30% of the fish meal control diet (crude protein 43%), cottonseed meal, canola meal (protein ratio of 1:1) composite, respectively, instead of 10%, 20%, 30% fish meal, and the other on this basis were added on lysine sulfate crystals methionine amino acid content of the crystal threonine with the control group, dubbed the the seven kinds nitrogen can feed, the Litopenaeus vannamei initial weight of 0.66 ± 0.01g. The experimental results showed that the experimental group of in Litopenaeus vannamei tail weight, weight gain, feed conversion ratio, specific growth rate, protein efficiency, survival rate difference is not significant; the Litopenaeus vannamei digestibility data analysis showed that the dry matter, The highest apparent digestibility of protein, fat control group, but with cottonseed meal, canola complex add group difference was not significant. Non-specific immunity analysis showed that the the shrimp serum PO activity and ACP activity differences among the groups significantly (p lt; 0.05), serum total protein content of the control group higher than the composite alternative group the ACP activity add crystalline amino acid substitution group than in the same level is not low added. Liver activities of SOD, GST, GSH, AKP, ACP activity control group was significantly higher than the alternative. Short, feed, cottonseed meal, double low rapeseed meal composite replacement of fish meal ratio is not higher than 30% (that is, the amount of feed cottonseed meal, canola meal complexes Add no more than 13%), the growth index with the control group, no significant difference. Dietary lysine sulfate, DL-methionine, crystal threonine promote the growth of shrimp little.

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