Study on the Pharmacokinetics of Tylosin-Amoxicillin Compound Medicine in Pig
|School||Northeast Agricultural University|
|Course||Basic Veterinary Science|
|Keywords||Ultra- high performance liquid chromatography - tandem mass spectrometry Amoxicillin Sodium Tylosin tartrate Tylosin tartrate - sodium amoxicillin in combination Pharmacokinetics Pig|
Tylosin tartrate - amoxicillin sodium indigenously developed combination drugs for the prevention and treatment of Streptococcus suis, pharmacokinetic study to guide its clinical application. Were established for the determination the pig serum tylosin tartrate and sodium amoxicillin ultra-high performance liquid chromatography tandem mass spectrometry, this method of determination of tylosin tartrate, amoxicillin sodium compound tylosin tartrate - amoxicillin blood concentration of sodium, according to the plasma concentration - time calculated pharmacokinetic parameters. Resulting pharmacokinetic parameters were statistically analyzed to compare the pharmacokinetic parameters of single drug and combination, to provide a theoretical basis for guiding the clinical application. Established efficient, sensitive and ultra-high liquid - tandem mass spectrometry pig serum concentration of sodium amoxicillin, serum samples by solid-phase extraction, acetonitrile -0.2% acetic acid (5:95, v / v) as mobile phase gradient elution using ACQUITY UPLCTM BEH C18 column by electrospray ionization tandem mass spectrometry to select multiple reaction monitoring ion monitoring. Ions for quantitative analysis of the reaction mass to charge ratio m/z366.3 GT; 349.2; the serum endogenous substances and other substances do not interfere amoxicillin sample peaks. The linear range of amoxicillin 0.06μg/mL ~ 1μg/mL, standard curve correlation coefficients (R2) of 0.9975; pig serum samples analysis precision were less than 10%; in 0.06μg/mL, 0.25μg/mL average recovery rate and the the concentration 1μg/mL three serum samples were 68.3%, 72.0% and 75.1%; quantitative concentration lower limit of 0.03 and. This approach is consistent with the requirements of the analysis of biological products, for amoxicillin sodium pharmacokinetic studies. 10 healthy pigs oral amoxicillin sodium (15mg/kg) and compound tylosin tartrate (10mg/kg) - amoxicillin sodium (15mg/kg) after the main pharmacokinetic parameters were Cmax (0.88 ± 0.01) and (2.13 ± 0.44) μg / mL. TP (0.92 ± 0.06) and (0.95 ± 0.16) h, the elimination of half-life T1/2β (0.78 ± 0.06) and (1.31 ± 0.96) h, the AUC (2.24 ± 0.11) and (5.81 ± 2.14) (μg / mL) * h. Statistical analysis of these pharmacokinetic parameters, tylosin tartrate single drug amoxicillin sodium and tylosin tartrate combination amoxicillin sodium pharmacokinetic parameters, expressed as: tartaric acid Thailand Lok cephalosporin enhance the degree of absorption of amoxicillin, but slow down the absorption rate of amoxicillin in vivo; maintained a transhipment of amoxicillin to the tissue, to maintain a higher blood concentration of the longer time; slowed amoxicillin elimination rate of the pigs, to extend the duration of action of amoxicillin in the body. Established efficient and sensitive ultra performance liquid - tandem mass spectrometry determination of the concentration of tylosin tartrate in swine serum pharmacokinetics of pig oral tylosin tartrate. Serum samples by solid phase extraction, acetonitrile -0.2% acetic acid (30:70, v / v) as the mobile phase gradient elution, using ACQUITY UPLCTM BEH C18 column by electrospray ionization tandem mass spectrometry to selected reaction monitoring ion monitoring. For the quantitative analysis of ion reaction for m/z916.9 GT; 772.4. The the serum endogenous substances and other substances do not interfere with the sample peak of amoxicillin. Amoxicillin the linear range of 0.5 ng / mL ~ 50ng/mL, standard curve correlation coefficients (R2) is 0.9994; pig serum sample analysis precision were less than 15%; 0.5ng/mL, 5ng/mL and The the 50ng/mL three concentrations of serum samples average recoveries were 74.0%, 78.4% and 83.3%; quantitative concentration lower limit of 0.5ng/mL. This approach is consistent with the analysis requirements of biological products can be used for the pharmacokinetic study of tylosin tartrate. 10 healthy pigs were oral tylosin tartrate (10mg/kg) and compound tylosin tartrate (10mg/kg) - amoxicillin sodium (15mg/kg) after the main pharmacokinetic parameters were Cmax was (27.41 ± 10.13) ng / mL (42.49 ± 20.65) ng / mL (33.56 ± 13.84) ng / mL (27.42 ± 13.37) ng / mL, Tp is (0.37 ± 0.09) h, (4.55 ± 0.93) h (0.33 ± 0.07) h, (4.87 ± 1.87) h, elimination half-life T1 / 2 (4.08 ± 0.67) h, (3.65 ± 0.74) h, AUC0-8 for (156.27 ± 35.63) (ug / mL) * h , (94.50 ± 23.95) (ug / mL) * h. Statistical analysis of these pharmacokinetic parameters, said 明阿莫西林 sodium does not affect the pharmacokinetic parameters of tylosin tartrate in pigs.