Dissertation
Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology

Experimental Study of Salvia to Prevent Calcium Oxalate Stones in Male Rats

Author KangRan
Tutor LuoZhiGang
School Nanhua University
Course Department of Urology
Keywords Salvia Male rats Calcium oxalate stones Osteopontin
CLC R285.5R
Type Master's thesis
Year 2010
Downloads 32
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Objective: ethylene glycol and ammonium chloride, induce the formation of calcium oxalate stones in rat models, and by detecting the rats 24h urinary oxalate concentration (Ox), calcium Ca2, blood urea nitrogen (BUN), creatinine (Cr ), calcium (Ca2), phosphorus (P), malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, renal tissue malondialdehyde (MDA) content, superoxide dismutase (SOD) vitality, histopathologic and immunohistochemical determination of osteopontin (OPN), a preliminary understanding of Salvia on renal calcium oxalate stones preventive effect and its mechanism. Methods: 24 outbred population (Sprague Dawley, SD) male rats were randomly divided into three groups, each 8: (a) control group, experimental normally fed pelleted feed and water, and intraperitoneal injection of the same amount of physiological brine; (2) induced rock group, experimental drinking water containing 1% ammonium chloride and 1% ethylene glycol solution of water and a normal pellet feed, and intraperitoneal injection of normal saline; (3) Salvia intervention group, the experiment drinking water containing 1% ammonium chloride and 1% ethylene glycol solution of water and a normal pellet feed, and daily intraperitoneal injection of Salvia Injection 2ml / kg / d. Two weeks later, rats were detected Ox 24h urine concentration, Ca2 concentration, serum BUN, Cr, Ca2, P, MDA content, SOD activity, renal tissue MDA content, SOD activity levels, kidney HE staining, light tubular cavity of each group were observed crystal stones and tubular dilatation, immunohistochemistry to detect the renal tissue expression levels of OPN. Results: 1 trap rock group and intervention group Salvia Ox 24h urine concentration, 24h urinary Ca2 concentration was significantly higher, the difference was significant (P lt; 0.05); Salvia intervention group compared with the lure stone, 24h urinary Ox Ca2 concentration and 24h urine concentrations were significantly decreased, the difference is also significant (P lt; 0.05). 2 lure rock group and intervention group Salvia serum BUN, Cr concentration was significantly higher, the difference was statistically significant (P lt; 0.05); Salvia serum Cr concentration in the intervention group was significantly lower than the lure rock group, the difference There were significant (P lt; 0.05); Salvia intervention group serum BUN concentration induced rock group, the difference was not significant (P> 0.05). Each group of serum Ca2, P concentration compared between the two groups showed no significant difference (P gt; 0.05). 3 Stone serum and homogenate induced superoxide dismutase (SOD) activity minimum, compared with the control group, the difference was statistically significant (P lt; 0.05); Salvia SOD activity in the intervention group compared to lure rock group, the difference There were significant (P lt; 0.05).; compared with the control group, the intervention group Salvia serum SOD activity was reduced without significant (P> 0.05), Salvia homogenate SOD activity intervention group were significantly lower significance (P lt; 0.05). 4 lure stone serum and malondialdehyde (MDA) in the highest compared with the control group, the difference was statistically significant (P lt; 0.05); Salvia intervention group compared with MDA induced rock group, and the difference was significant significance (P lt; 0.05); compared with the control group, serum MDA Salvia intervention group were significantly higher (P lt; 0.05), the difference was statistically significant, Salvia intervention homogenate MDA in rats no significant increase significance (P> 0.05). 5 tubules normal control group, induced a large stone set into a piece of tubular cavities visible presence of calcium oxalate crystals, lumen expansion; Salvia intervention group tubular cavities scattered presence of calcium oxalate crystals, a few flaky, luminal mild expansion. Crystal stones crystal stones rating prompted SM group was significantly reduced compared to a rock group, the difference was statistically significant (P lt; 0.05). 6 immunohistochemical results suggest that in the control group could be detected weak expression of OPN, induced stone renal tissue of OPN expression was significantly increased. Salvia intervention renal tissue expression of OPN induced rock group below. OPN expression intensity scores prompted SM group OPN protein expression decreased compared to stone formation, the difference was statistically significant (P lt; 0.05). Conclusions: 1. Salvia can make drinking induced rat kidney stones agent to reduce the formation of calcium oxalate stones. 2 Salvia possibly through antioxidant response, reduce free radical damage to renal tubular epithelial cells, while reducing the consumption of agents in rats induced calcium oxalate stone formation of stones. 3 Salvia may also be able to reduce the expression of osteopontin to reduce drinking in rats induced calcium oxalate stone formation agent.

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