Study on Relation between the Expression of S-100β& MBP and Traumatic Subarachnoid Haemorrhage in Brain of the Acute & Chronic Alcoholism Rats
|Keywords||Forensic pathology TSAH Alcoholism S-100β MBP|
Background and Purpose traumatic subarachnoid hemorrhage (traumatic subarachnoid haemorrhage, TSAH) a different brain contusion caused by subarachnoid hemorrhage, subarachnoid hemorrhage and pathological independent subarachnoid hemorrhage, and trauma sexual epidural hemorrhage, subdural hemorrhage tied for the three-traumatic intracranial lacunar hemorrhage. , Scholars reported TSAH often associated with alcohol-related, but the research reports of its occurrence and the mechanism of death is rare. Therefore, the discussion and interpretation of acute and chronic alcoholism the TSAH occurrence and death mechanisms can be analyzed for their cause of death, judicial trial sentencing scientific theory. The preliminary work of our group has successfully established a rat of acute and chronic alcoholism TSAH of model, results show slight concussion sexual the external force to combat alcoholism rats TSAH occurred rate: 82.4% of the chronic group, 28.6% of the acute group, the mortality rate : chronic group 58.8%, 0% of the acute group, the chronic alcoholism groups TSAH incidence and mortality than acute alcoholism group. And MMP-9 vascular wall of toxic substances, blood vessel wall morphology and biomechanics of Ngb, Hif-1α, EPO, Na ~~, K ~-ATPase and other brain oxygen and energy metabolites, tPA, Cyt-C of Caspase- Caspase-3, Bcl-2, Bax and other apoptosis related substances, confirmed the of alcohol toxicology role TSAH occurrence and death mechanisms play an important role. The experiment on this basis, to further explore the relationship of chronic alcoholism in rats TSAH high mortality rate and changes in glial cells, select is closely related to the functional structure of the central nervous fiber glial cell-specific markers S-100β MBP Discussion of the brain tissue in the case of acute and chronic alcoholism the expression of the S-100β, MBP, and its correlation with rat TSAH death mechanisms. Materials and methods Animal model and grouping SPF adult male SD rats 61, weight 300 ± 50g, sub cage adaptability fed for two weeks, and randomization. 1.1 28 rats were randomly acute alcoholism model the sub acute the simple Guanjiu group, the acute alcoholism blow group, the acute simple irrigation group, irrigation insult group of four groups, each group of seven. Alcoholism group disposable liquor (Beijing Red Star winery Erguotou, 52% v / v) gavage 15ml/kg; irrigation group the same dose of drinking water orally. Insult group 2h irrigation liquor or potable water, concussion blow. 1.2 the chronic alcoholism Model 33 rats were randomly divided into chronic alcoholism group alone seven, chronic alcoholism stopped drinking combat group of seven, chronic alcoholism blow group of 19 (including 10 classified as death death group survived 9 classified as a survival group) 4 group. Each group of rats given alcohol toxicity (Beijing Red Star winery Erguotou, 52% v / v), twice a day, eight hours apart, two weeks before each time I gavage doses 8ml/kg two weeks after each gavage dose to 12ml/kg, last alcoholism 2h, chronic alcoholism stopped drinking combat group in alcoholism after 12h, concussion blow. 2 each alcoholism to combat and irrigation insult group rats concussion combat, placed 4h to ether anesthesia, thoracotomy left ventricular blood 5ml the the death rat immediately thoracotomy blood 5ml inserted through the left ventricle - aortic tube were sacrificed and fixed brain tissue perfusion of 4% paraformaldehyde solution craniotomy to take the whole brain, placed in 4% paraformaldehyde fixed, drawing, embedded in paraffin, HE sections were stained, solid green (Fast green ) the myelin staining brain tissue and myelin tissue morphology, S-100β and MBP immunohistochemical staining, Image-Pro Plus 6.0 image analysis software stereological quantitative histopathological observations, BAC testing. All data application SPSS15.0 statistical software, t-test and single-factor analysis of variance, chi-square test statistical analysis. Results of acute and chronic alcoholism rat behavior performance and the the BAC changes in acute alcoholism group 0.5h within activities increased in the rats alcoholism, stop looking, was excited state after reduced activity, unsteady gait, clumsiness, was drowsiness, slow breathing, unresponsive drunken performance. Behavior and blood pressure were alcoholism after the 2h blood alcohol concentration (BAC) of 180.16 ± 16.58 mg / dL, and blood pressure 14.13 ± 0.34 Kpa, blood pressure than alcoholism 17.02 ± 0.88 Kpa significantly decreased (P lt; 0.01); irrigation group was given no significant change (P gt; 0.05). The during chronic alcoholism rats Guanjiu the, the gradual emergence of weight loss, malnutrition, reduced food consumption, listlessness, and part of the side of hemiplegia Chronic Alcoholism change, 307.2 ± 28.9g 4w after weight 251.8 ± 19.6g than orally before obvious decreased (P lt; 0.01), blood pressure 18.16 ± 0.82Kpa 16.60 ± 0.76Kpa than gavage was significantly higher (P lt; 0.01). Within 0.5h after alcoholism was side, drowsiness, turn the acute alcoholism performance is reflex Last alcoholism after 2h BAC 208.50 ± 32.35 mg / dL above the acute group. 2 TSAH incidence, mortality and pathology observed acute alcoholism against rats TSAH incidence of 28.6%, with no deaths, irrigation blow the group no TSAH and death; the chronic alcoholism strike group TSAH incidence of 84.2% mortality rate of 52.6%, chronic alcoholism stop the the wine against group TSAH rate of 71.4%, 28.6% mortality, mortality slower alcoholism insult group were significantly different (P lt; 0.01). Acute simple irrigation group the scattered brain surface in limitations congestion; acute irrigation to combat rats brain surface is smooth and was ceroid pale; acute mere alcoholism group rat frontal and cerebellar surface large areas of congestion; the acute alcoholism combat group occurred TSAH large surface of the mouse brain and brainstem dorsal, ventral patchy thin layer of SAH; the chronic alcoholism blow group TSAH the rat whole brain diffuse thick layer of SAH heavy ventral surface of the brainstem. Histological examination, acute alcoholism group of neurons and glial cells with mild water degeneration, cell week gap mild expand the combat group brainstem and cerebellum thin layer of SAH. Disordered arrangement of layers of neurons of the cerebral cortex of chronic alcoholism group, reducing the number of nuclear pyknosis and common neuronophagia phenomenon; the cerebellum Po Ken the wild cells osteoporosis is less cell body triangle pyknosis smaller processes to reduce nuclear pyknosis partially dissolved disappeared; the medulla more common homogenized cytoplasm deeply stained pyknotic nuclear pyknosis dark neurons (Dark cell); the combat group brainstem, frontoparietal, and cerebellum blockbuster thick layer of SAH. Solid green myelin staining (myelin dark green, red nucleus), acute simple irrigation group brainstem, corpus callosum and brain fiber myelin straight, tightly packed; of acute alcoholism blow group myelin arranged slightly loose, the gap , coloring is paler; a chronic strike group myelin loose, the gap increases, uneven coloring pale brainstem can be seen clearly irregular thickening distorted fracture nerve fibers week gap was significantly expanded. 3 S-100β protein expression, MBP 3.1 S-100β protein-positive cells in the number of acute group of alcoholism blow and irrigation to combat rats S-100β positive cells in various brain regions were significantly more acute alcoholism and acute simple irrigation group ( P lt; 0.05); blow between groups were not statistically different between the simple group (P gt; 0.05). The chronic alcoholism combat survival group and chronic alcoholism alcoholics stop drinking, to crack down on the number of positive cells of the brain regions surviving rats were significantly more than chronic simple alcoholism group and chronic alcoholism combat the death group (P lt; 0.05); chronic alcoholism blow survival group and chronic alcoholism stopped drinking combat between the survival of the group, the the of Chronic Simple alcoholism group and chronic alcoholism combat between the death group had no significant difference (P gt; 0.05). The chronic alcoholism combat survival group and chronic alcoholism combat survival positive cells in various brain regions of rats stopped drinking significantly more than the acute group (P lt; 0.05); chronic simple alcoholism group and chronic alcoholism combat death group each The brain regions were more than the number of positive cells in acute pure alcoholism and acute simple irrigation group (P lt; 0.05), and of acute alcoholism blow and irrigation to combat group, no significant difference (P gt; 0.05). 3.2 S-100β protein IOD value the acute alcoholism combat group and irrigation to combat group brain regions the S-100βIOD value were higher than acute simple the alcoholism group and acute simple irrigation group (P lt; 0.05) between the insult group, simple group between no statistical difference (P gt; 0.05). Chronic alcoholism group alone, the highest IOD values ??are significantly higher than that of other chronic alcoholism group (P lt; 0.05); the chronic alcoholism combat IOD value of the minimum of the death group were significantly lower than in other chronic alcoholism group (P lt; 0.05); the chronic alcoholism combat survival group with chronic alcoholism stopped drinking IOD values ??against the survival group had no significant difference (P gt; 0.05). In addition to the chronic alcoholism combat other than death group, the IOD values ??of other chronic alcoholism group are higher than the acute group; the chronic alcoholism combat IOD value of the group of death and acute alcoholism combat and irrigation blow group no significant difference (P gt; 0.05) than acute alcoholism and acute simple irrigation group (P lt; 0.05). 3.3 MBPIOD value of acute alcoholism combat group and acute irrigation blow group brainstem and corpus callosum MBP IOD values ??were significantly lower than alcoholism and simple irrigation group (P lt; 0.05), to combat groups simply between groups was not statistically difference (P gt; 0.05); the cerebellum among groups there was no significant difference (P gt; 0.05). The chronic alcoholism simply group all brain regions MBP IOD highest than other chronic alcoholism insult group (P lt; 0.05); corpus callosum and cerebellum MBP IOD values ??between each of chronic alcoholism blow group had no significant difference (P gt; 0.05); chronic alcoholism to combat the death group brainstem MBP IOD value is higher than the the chronic alcoholism combat survival group and chronic alcoholism stopped drinking combat survival group (P lt; 0.05) between the survival group, no significant difference (P gt; 0.05 ). No significant differences in rat brain regions MBP IOD value addition to Chronic Simple Guanjiu group with acute blow group (P gt; 0.05), chronic alcoholism group the brain area MBPIOD values ??are lower than the acute group (P lt; 0.05). Conclusion 1, acute and chronic alcohol abuse can be synergistic concussion blow precipitating TSAH chronic alcoholism TSAH to death an important factor. 2, chronic alcoholism, brain organization of glial cells in S-100β protein expression enhanced nerve fiber myelin MBP decreased expression may be involved in chronic alcoholism alcoholic brain atrophy and alcoholic encephalopathy mechanism and constitute Chronic the alcoholism TSAH death mechanism material basis.