Study of High Density and Topping Cultivation Technique in Hyacinth Bean and Influence on the Content of Isoflavones by Interferencing the Flavone Synthase Gene
|School||Shanghai Jiaotong University|
|Course||Crop Genetics and Breeding|
|Keywords||Lablab purpureus High-density Cultivation Topping Glycine max Isoflavones Agrobacterium Rhizogenes|
Lablab purpureus, which is the vegetable of extensive use, enormous economic value and bright application prospect, has been on industrialization gradually. Accompanied with industrialization, the ever-increasing demand for quality and yield pushes L purpureus planting technique to innovate. The research focused on exploring and analyzing L purpureus planting technique on both planting densities and topping. The cultivar, named‘Shanghai Jiaoda Red Lentil No.1’, was investigated for the yield under different planting densities. An appropriate planting pattern for increasing the L purpureus production was determined by yeild evaluation under 4 planting densities in 2 picking stages, which also provided a theoretical basis for its high density cultivation. The results indicated that the highest yield was the planting density 49995 plants/hm2. In the experiment, the yeild increased 124.3% compared with traditional cultivation pattern, when its row distance and plant distance was 2 m and 0.2 m respectively, and planting density was 49995 plants/hm2. The yield increased 80.48%~272.96% under high density cultivation at the first stage (April 15th to June 30th), and 0~9.8% at the second stage (July 1st to October 15th), which meaned that the effect of high density cultivation was obvious on the first stage was better than that of the second stage. The yield can be improved through reasonable high density cultivation in the production of hyacinth bean. The impact of agricultural traits and yield under L purpureus topping planting was investigated with the cultivar‘Shanghai Jiaoda Red Lentil No.1’. L purpureus topping processes were at the stages of 2-3 leaves, 4-5 leaves, 6-7 leaves and 8-9 leaves without trellis, and their height were controlled at 40cm, 60cm, 80cm, 100cm respectively . The suitable L purpureus topping technique and theoretical basis for L purpureus erection cultivation were provided via analyzing of lodging degree, blossom period, harvest time, fresh weight per plant, fresh pod yield per plant and economic coefficients. The results indicated that the suitable topping process was at the stage of 2-5 leaves with 1.2~2.3 lodging degree, 7.3~9.6 days earlier blossom period, 4.6~6.6 days earlier harvest time, which was significant differences with control group, and stem fresh weight at 846~1083g per plant, a decrease of 42% ~25.8% compared with control group; fresh pods number decrease 10.4~20.7 per plant and fresh pod yield decrease 47~137g per plant compared with CK. While the economic coefficients increased 0.131 under the topping treatment at the stage of 2-5 leaves . Consequently, in production of Lablab purpureus, taking topping process at the right time with high-density cultivation techniques could improve the growth and economic benefits effectively.Isoflavone is a sub-category in flavonoids family, which is generated a class of secondary metabolites in the plant and play a very important role in plant growth and human health. There are mainly 12 kinds of isoflavones in soybeans. Compared to the low efficiency of traditional breeding, using control phenyl propionic acid class of metabolic pathways to improve the content of isoflavones in soybeans has a significant advantage in avoiding time-consuming and improving the productive potential. In the study the flavonoid synthase gene was interferred in soybean (var. Kenfeng 16) by Agrobacterium Rhizogenes K599 and ATCC15834 transformation After GUS histochemical detection and PCR molecular identification, the study screened out transgenic hairy root, and the content of isflavonoids was analysed through HPLC (high performance liquid chromatography). We obtained transgenic hairy root, but also found species of Agrobacterium Rhizogenes, plant receptor genotype, and the related method of operation have a large impact on the conversion rate. K599’s conversion rate is higher than that of ATCC15834. HPLC analysis showed that in the transgenetic hairy root, the content of Daidzin, Glycitin, Genistin, Daidzein, Genistein amounted to 581.9 ?g/g, 9.3 ?g/g, 23.3 ?g/g, 43.5 ?g/g, 1.6 ?g/g compared with the control group, and the growth rate were 68.1%, 66.9%, 41.0%, 40.4% and 37.2%, and totally increased 659.6 ?g/g, and growth rate was 63.6%.