The Expressions and Significance of MMP-2 and TIMP-2 in Skin of Inguinal Hernia
|Keywords||Inguinal hernia Matrix metalloproteinase-2 Tissue inhibitor of metalloproteinase factor-2 Reverse transcriptase polymerase chain reaction|
Background and purpose of inguinal hernia is the the abdomen most common surgical diseases. However, because the incidence of inguinal hernia, especially adult inguinal hernia causes not very clear. Over the years, people from anatomy (groin area is weak) and dynamics (intra-abdominal pressure) angle to analyze the reasons for the formation of inguinal hernia, most scholars believe, the groin area transverse fascia weaknesses or defects adult inguinal hernia occurs the main reason. In recent years, scholars from biochemistry to study the causes of inguinal hernia, the doctrine of collagen metabolism: reduced collagen type I collagen type III relative increase Ⅰ / type III collagen ratio decreases, resulting in a weak transverse fascia of the groin area or defect caused by the occurrence of inguinal hernia. The metabolism of collagen by matrix metalloproteinases (matrix metallo-proteinases, MMPs) and their inhibitors (tissue inhibitor of matrix metalloproteinases, TIMPs) adjustment. MMPs and TIMPs play an important role in maintain stability and structural integrity of the ECM. MMPs synthesis and secretion by fibroblasts, participate in Ⅰ metabolism of type III collagen fibers, is a group of Zn2 dependent endopeptidase can degrade almost all components of the ECM. Has found that The MMPs family has more than 20 species, with a high degree of homology. Discovered in recent years that MMPs in natural suppressor factor inhibitors - TIMPs, MMPs play an inhibitory effect irreversible binding, preventing collagen degradation, and play an important role in normal tissue reconstruction, as well as a variety of pathological processes. TIMPs has a strong affinity with MMPs inhibitors - TIMPs binding and activation of MMPs by 1:1, so as to effectively prevent the binding of MMPs and substrates, resulting MMPs inactivation. Seen between MMPs and TIMPs affect collagen metabolism may play a role in the occurrence and development of inguinal hernia. So far, MMPs and TIMPs inguinal hernia experimental study reported very little, and the findings need to be further confirmed. This experiment is the application of reverse transcriptase polymerase chain reaction method (Reverse transcript polymerase chain reaction, RT-PCR), comparative analysis of the major enzymes involved in collagen metabolism MMP-2 and TIMP-2 in the adult inguinal hernias and hernia patients skin explore the relationship of inguinal hernia affect collagen metabolism enzyme expression from biochemistry. Provide a theoretical basis for the study of the etiology of inguinal hernia, and provide theoretical guidance for clinical treatment. Materials and methods The experiments with the experimental group and the control group drawn from the two groups were abdominal skin inguinal hernias and hernia patients, because MMPs and TIMPs mainly by the fibroblasts synthesize and secrete skin fibroblast most rich. Experimental group: inguinal hernia and 50 patients, including 31 cases of indirect inguinal hernia, direct hernia 19 cases, drawn from the implementation of primary inguinal hernia repair surgery patients from June 2009 to November 2009, the First Affiliated Hospital of Zhengzhou University, Zheng. Control group: no 10 cases of inguinal hernia patients, based on the experimental group at the same time the same hospital for lower abdominal surgery patients. The two sets of material positions are under the skin of the abdomen. Detected by RT-PCR the expression of of MMP-2mRNA and TIMP-2mRNA, skin. SPSS13.0 statistical software for statistical analysis of the experimental data. Measurement data compared using one-way ANOVA, two sample comparison using two-way t-test, correlation analysis using linear correlation. P lt; 0.05 was considered statistically significant. Results of the experimental and control groups were visible the MMP-2mRNA, TIMP-2mRNA stable expression. Experimental group (direct hernia) and higher levels of MMP-2 expression (p = 0.025 lt; 0.05), lower level of TIMP-2 expression (p = 0.031 lt; 0.05), compared with the control group, the differences are statistically significant. The experimental group (oblique hernia) compared to the expression levels of MMP-2 mRNA and of TIMP-2mRNA the control group had no significant difference (PMMP-2 = 0.055 gt; 0.05) (PTIMP-2 = 0.073 gt; 0.05). 3 compared with the non-long-term smoking group, long-term smoking group higher expression levels of MMP-2mRNA (p = 0.023 lt; 0.05), lower level of TIMP-2 expression (p = 0.041 lt; 0.05), the differences were statistical significance. The expression levels of MMP-2mRNA age 4 skin was a positive correlation (r = 0.227, P = 0.041 lt; 0.05), its expression levels increase with age. The expression levels of TIMP-2mRNA age was negatively correlated (r = -0.372, P = 0.014 lt; 0.05), reduce its expression level increases with age. The 5 skin the MMP-2mRNA and of TIMP-2mRNA the expression level was negatively correlated (r = -0.629, P = 0.037 lt; 0.05), With the TIMP-2mRNA expression levels, the expression levels of MMP-2 reduced. Conclusion 1 MMP-2mRNA direct inguinal hernia patients was The high expression and TIMP-2mRNA, showed low expression showed that MMP-2 and TIMP-2 is involved in the metabolism of collagen, the straight with primary inguinal hernia formation. 2 MMP-2mRNA and TIMP-2mRNA, no significant difference in the expression level of the inguinal hernia patients with the control group, indicating that no significant relationship between the formation of both indirect inguinal hernia. 3 long-term smoking patients showed a high expression of MMP-2mRNA, TIMP-2mRNA showed low expression, that smoking cigarettes may be one of the reasons for inguinal hernia. 4 MMP-2mRNA expression with age was positively related and TIMP-2mRNA, expression with age was negatively correlated, indicating that the older the higher the incidence of inguinal hernia. 5 MMP-2mRNA was negatively correlated with the expression levels of TIMP-2mRNA that TIMP-2 inhibits the expression of MMP-2, and the regulation of collagen metabolism involved in the occurrence of inguinal hernia.