The Research on Analgesic Mechanism of Dragon’s Blood and Its Components - Loureirin B
|School||Central South University for Nationalities|
|Keywords||Dragon's Blood Loureirin B Capsaicin Sodium channel Patch clamp|
The Dai medicine Longxuejie is blindly traditional medicines, both blood circulation and styptic effect, and has significant analgesic and anti-inflammatory effects, so widely used in clinical practice, the last few years, the experimental group Longxuejie of The analgesic mechanism to do more research, through the patch-clamp technique in rat primary sensory neurons (DRG and TG neurons) study Longxuejie TTX-S and TTX-R sodium channel current and chili receptor currents. Also extracellular microelectrode recording technique, the Dragon's Blood and its components on the corner of wide dynamic range of spinal cord dorsal nerve element induced by the impact of the discharge, the above findings illustrate the Dragon's Blood from different levels, different mechanisms of modulation The pain information hub incoming, so as to achieve the analgesic effect. Dragon's Blood Dragon's Blood in rat dorsal root ganglion cells, sodium channel modulation and Longxuejie of inhibition of rat spinal dorsal angle wide dynamic range neurons evoked discharge frequency information through the central and peripheral pain modulation, but the lack of the analgesic activity types Longxuejie experimental study. Before the study the the Longxuejie play its analgesic effect through the intervention of pain information to block nerve conduction, DRG cell body axon of a branch of the sciatic nerve and peripheral connected to the research, if partial sciatic nerve, Longxuejie block nerve conduction is even more convincing. Therefore, this article first of a series of pharmacological experiments on mice, with hot plate, thermal radiation and writhing to determine the analgesic Longxuejie activity type, found through experiments, 0.5 g / L of dragon's blood dried in the hot plate test and experiment in light and heat radiation tail flick method can significantly prolong the the mice licking latency and tail-flick latency; mouse writhing experiment, you can significantly reduce 15min writhing times. Experimental results show that Longxuejie both good analgesic activity, also have good outer peripheral analgesic activity. Secondly, the pharmacological mechanism of the analgesic effect to verify Longxuejie produce, this article will be produced bullfrog sciatic nerve specimen, to observe Longxuejie the sciatic nerve action potential. Found through experiments, 0.5 g / L Longxuejie makes bullfrog sciatic nerve action potential amplitude lower the nervous excitement refractory period and excited conduction velocity, these results further identified Longxuejie block nerve conduction, also provide further evidence for the Dragon's Blood on the intervention of the pain signal transduction. In addition, by patch clamp experiments have found that Longxuejie the main ingredient Loureirin B of the closely related pain passed sodium channel and capsaicin receptor are modulated been reported in the literature, capsaicin able modulation of voltage-gated sodium channels, inhibition of sodium current, and this modulation of the capsaicin receptor and capsaicin has Loureirin B similar chemical structure Loureirin B, the sodium channel current inhibition thus speculated of B on Loureirin sodium channel current impact is likely to be related to the capsaicin receptor. Clarify the results obtained will be more valuable, based on the above analysis, the paper design patch-clamp experiments to conduct in-depth study of the analgesic mechanism of the Dragon's Blood and its components Loureirin B. Based Loureirin B similarity with capsaicin in modulation capsaicin receptor and sodium channel. Finally, using the whole-cell patch clamp technique, B modulation Loureirin sodium channels with capsaicin receptor relationship in DRG neurons, and to explore the interaction between B and capsaicin Loureirin. Through experiments the following results: 1, B and capsaicin Loureirin combination of sodium current inhibition rate than separate role higher; 2, B and capsaicin Loureirin interaction, when fixed any of them the concentration of the drug, and changing the concentration of another drug, the degree and acceleration sodium channel will be the extent of deactivation of the activation curves in the depolarizing direction offset with increasing drug concentration decreases; 3, by Drug Interactions Analysis the come Loureirin B and capsaicin modulation of sodium channel antagonism. These results also show that the to B on Loureirin sodium channel modulation effect is indeed related to the capsaicin receptor B and capsaicin Loureirin sites is not the same receptor with a point, there is no competition between the two sexual antagonism. The results of this study can be used as the analgesic effect of Longxuejie new mechanism and prior to combination of the analgesic Longxuejie findings, Dragon's Blood, we are able to carry out in-depth pharmacological studies, and the development of new town next pain drugs, but also conducive to the secondary development of the Dragon's Blood, improve their scientific and technological content, and expand the social and economic benefits.