Dissertation
Dissertation > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Islet disease > Diabetic coma and other complications

Effects of Angiopoietin-1 on Vascular Angiogenesis Regulator Factor in Human Umbilical Vein Endothelial Cells Cultured with High Glucose

Author WeiZuoZuo
Tutor YangYiBin
School Zunyi Medical College,
Course Internal Medicine
Keywords high glucose human umbilical vein endotheliai cells Angiopoietin PEDF VEGF
CLC R587.2
Type Master's thesis
Year 2011
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Objective:To observe the expressional changes of angiopoietin-2 (Ang-2), pigment epithelium-derived factor (PEDF) and vascular endothelial growth factor (VEGF) in human umbilical vein endothelial cells (HUVECs) cultured with high glucose, and i nvestigate the effects of angiopoietin-1 (Ang-1) on these factors in high glucose.Methods:The HUVECs were divided into the following groups:normal group (5.5 mmol/L glucose), mannitol group (5.5 mmol/L glucose+24.5 mmol/L mannitol), high glucose group (30 mmol/L glucose) and Ang-1 group (30 mmol/L glucose+250 ng/ ml Ang-1). The expression of Ang-2, PEDF and VEGF were detected by immunofl uorescence, immunohisochemistry and real-time polymerase chain reaction (real-time PCR) at 24 and 48 hours after treated with different methods.Results:(1) Ang-2 was mainly expressed in the HUVECs cytoplasm and (or) membrane. The protein and mRNA of Ang-2 between the normal and mannitol group were not statistically different (P> 0.05); The levels of Ang-2 protein and mRNA in high glucose and Ang-1 groups were increased with time dependency, but significant difference were only found at 48h in high gloucose group (P<0.05); In the Ang-1 group, the levels of Ang-2 protein and mRNA at 48h were lower than that in high glucose group (P<0.05).(2) PEDF was mainly expressed in the HUVECs cytoplasm and (or) membrane. The protein and mRNA of PEDF between the normal and mannitol group were not statistically different (P>0.05); The levels of PEDF protein and mRNA in high glucose and Ang-1 groups were lower than that in the normal group (P<0.05), and the levels of PEDF in high glucose group were decreased with time dependency (48h vs 24h,.P<0.05); Compared to high glucose group, the increased levels of PEDF protein and mRNA in Ang-1 group were not statistically different (P> 0.05).(3) VEGF was mainly expressed in the HUVECs cytoplasm and (or) membrane. The protein and mRNA of VEGF between the normal and mannitol group were not statistically different (P>0.05); The levels of VEGF protein and mRNA in high glucose and Ang-1 groups were higher than that in the normal group (P<0.05), and the levels of VEGF in high glucose group were increased with time dependency (48h vs 24h, P<0.05); Compared to high glucose group, the decreased levels of VEGF protein and mRNA in Ang-1 group were not statistically different (P> 0.05).Conclusion:High glucose can induce abnormial expression of Ang-2, PEDF and VEGF in HUVECs in vitro, and may be with time dependency; Exogenous administration of Ang-1 can alleviate the abnormal expression of Ang-2, PEDF and VEGF in HUVECs cultured with high glucous.

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