Dissertation
Dissertation > Industrial Technology > Machinery and Instrument Industry > Instruments, meters > Medical and health devices > Diagnostic test instruments used

Fluorescence quantitative PCR Detection System

Author YaoYingHao
Tutor ChenZhangWei
School Zhejiang University
Course Mechanical and Electronic Engineering
Keywords Quantitative PCR Fluorescence detection PMT Signal Processing
CLC TH776
Type Master's thesis
Year 2011
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Polymerase chain reaction (PCR) amplification of a large number of in vitro specific DNA fragments , since 1985 since the invention in disease diagnosis, drug screening , inspection and quarantine areas such as access a wide range of applications , greatly promoted the molecular biology and other related disciplines development . Not only a large number of quantitative PCR amplification DNA, but also can achieve the accurate quantification of initial DNA template , making genetic analysis techniques from the original extensive qualitative analysis to quantitative analysis of the precise changes in the clinical diagnosis and other aspects of the inspection and quarantine significance . PCR fluorescence detection system by monitoring process , the fluorescence signal real-time conversion into electrical signals, an analysis and processing to achieve the initial template quantification of DNA , the quantitative PCR instrument to achieve accurate quantification of the core components from the fluorescent light-emitting principle herein , fluorescence quantitative PCR and quantitative principle start , through quantitative PCR experiments commonly used fluorescent labeling substance emitting principle , spectral characteristics analysis and research , quantitative PCR instrument for fluorescence detection of specific application requirements , combined with fluorescence analysis of various fluorescence detection methods, completed the fluorescence signal detection system designed and built to achieve a single excitation light at four different wavelengths on the fluorescence signal acquisition and processing . In addition, fluorescence detection system designed in this paper for the relevant performance tests. Experiments show that the system of background noise, high sensitivity, dynamic range, effectively shading , anti-interference ability , good repeatability, system performance basically meet the requirements of practical application . From an engineering point of view put forward both in the practical application of the system 's recommendations and system performance improvements, and future work are discussed.

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