Dissertation
Dissertation > Medicine, health > Oral Sciences > Oral medicine > Caries in teeth diseases

Experiment Study on the Effect of Hybrid Coat in Closing of Dentinal Tubules

Author MaZuo
Tutor WuPeiLing
School Xinjiang Medical University
Course Clinical Stomatology
Keywords Hybrid Coat Dentine hypersensitivity Dentinal tubules Crown tooth preparation SEM
CLC R781.2
Type Master's thesis
Year 2011
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Objective: This study aims to investigate the difference between two type of dentinal desensitization agent, (Gluma desensitization agent, Hybrid Coat desensitiza- tion agent) through the observation of experimental dentinal blocks after dental preparation in vitro under the scanning electron microscopy (scanning electron micros- copy, SEM), and to evaluate the obturated effect of the Hybrid Coat on dentinal tubules, confirming the protective effect of Hybrid Coat and giving a basis for clinical apply. Methods: Collected 20 intact extracted premolar tooth for orthodontic reason, were divided into buccal and lingual segment along the central groove of the occlusal surface, and buccal segments were divided into two parts along the buccal triangle ridge further. Then, every specimen was divided into three groups. group A: the lingual cusp; group B: the mesial buccal inclined surface; group C: the distal buccal inclined surface. After using the turbines with cool water to prepare every specimens for routine porcelain-fused-to-metal (PFM) requirements, cutting the root, grinding the enamel of the occlusal surface to expose the detine, and puting them in the ultrasonic cleaning machine with saline water for 10 minutes to remove little scrap off the tubes, three groups with group A: non-treatment group; group B with Gluma coating agent as control group, Group C with Hybrid coat for the test group were observed for the situation of the dentinal tubules under the scanning electron microscopy(SEM), measured the average diameter, density, and the relative area of the dentinal tubules by image-proplus 6.0 image analysis software. Finally, statistical analysis was carried out. Results: The average diameter of dentin tubular three groups compared between overall statistically signifi- cant, F=14.841, P=0.000. Gluma group of dentin tubular average diameter was significantly lower than the blank control group, P=0.000. Hybrid Coat group of dentin tubular average diameter was significantly lower than the blank control group, P=0.007. Hybrid Coat group of dentin tubular average diameter is obviously lower than that of the Gluma group, P=0.034. Dentin tubular, the density of the three groups more overall statistically significant, F=154.71, P=0.000. Gluma group of dentin tubular density was significantly lower than the blank group, P=0.000. Hybrid Coat group of dentin tubular density was significantly lower than the blank group, P=0.000. Hybrid Coat group of dentin tubular density was significantly lower than the Gluma group, P=0.004. Dentin tubular group compared the relative area of three general statistically significant, F=122.98, P=0.000. Gluma group of dentin tubular relative area was significantly lower than the blank group, P=0.000. group of dentin tubular relative area was significantly lower than the blank group, P=0.007. There was no significant difference in the relative area of dentin tubular in the Gluma group and Hybrid Coat group, P=0.659. Conclusion: This experiment through coating the Hybrid Coat and Gluma desensitization agent, observed dentinal blocks by the scanning electron microscopy, Hybrid Coat group was significantly lower than the Gluma group (P<0.05) in the average diameter and density. There was no significant difference in the relative area of dentin tubular in the Gluma group and Hybrid Coat group. It Showed that the Hybrid Coat in decreasing dentin tubular of density and average diameter were better than Gluma group. Through the observation of experimental, Hybrid Coat can make a certain effect in the aspect of closing dentinal tubules. So it can provides reference basis for the clinical application.

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