Dissertation > Industrial Technology > General industrial technology > Materials science and engineering > Special structural materials

Study on the Applications of Gold Nanoparticles in Capillary Electro-phoresis and Spectroscopic Analysis

Author ZhangMengJuan
Tutor ZuoHongTao
School Northwestern University
Course Chemistry
Keywords Gold nanoparticle Human serum albumin Bovine serum albumin Melamine Mercury ions Capillary Electrophoresis Laser thermal lens spectrometry
CLC TB383.1
Type Master's thesis
Year 2011
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Gold nanoparticle has a unique nature and effect, has to be applied in the analytical chemistry research. In this paper, the gold nanoparticle modified the inner surface of the capillary, the protein separation; gold particles used in absorption spectrometry, respectively, were measured of melamine and mercury ions. The article consists of the following four parts: Chapter Summary overview of the nature of the gold nanoparticles, preparation methods, and in recent years gold particles in the method of chemical analysis and based on the content of this thesis . Chapter II gold nanoparticle-modified capillary electrophoresis separation of proteins by physical adsorption gold particles modified fused silica capillary surface, determined by modified capillary electro-osmotic flow separation of the two similar proteins. The results show that the gold-modified capillary tube can be effectively suppressed electroosmotic flow and protein adsorption on the capillary wall, so that the separation efficiency is improved. Optimized experimental conditions, the two proteins of human serum albumin and bovine serum albumin to achieve a baseline separation. Concentration between the peak area showed a good linear relationship, the correlation coefficients were 0.9848 and 0.9943, and the detection limit for 0.83μg/mL and 0.80μg/mL (S / N = 3). Chapter Au probe laser thermal lens spectrometry method for the determination of melamine in research experiments to establish a new method of determination of melamine based on gold nanoparticle the spectral probe laser thermal lens spectroscopy. The presence of melamine, gold solution distinct color change occurs when the laser thermal lens spectrometry, using this phenomenon can achieve the purpose of determination of melamine in milk products. The results show that this method has a high detection sensitivity, the lowest detection limit of 5.8 ng / mL in the 0.12-1.0μmol / L within the signal strength with melamine concentration presents a linear relationship. Experimental determination of melamine in milk products spiked recoveries between 96.0 to 102.6%, the relative standard deviation of between 2.6 to 3.2%. Chapter cysteine ??functionalized gold probe laser thermal lens spectrometry determination of mercury ion (II) research study based on cysteine ??functionalized gold nanoparticle absorption spectroscopy for the determination of mercury ion (Hg2) method. Cysteine ??functionalized gold nanoparticle with amino, easy, and mercury ions in the aqueous solution of the ligand occurs, causing aggregation of gold nanoparticles, the color change occurs to a linear relationship, and in a certain concentration range. Cysteine ??functionalized gold nanoparticle-based color changes, study the establishment of a new method for determination of Hg2 a new laser thermal lens spectrometry method detection limit for 0.0349μg/mL. Experimental determination of Hg2 content of water samples using this method and recovery experiments. Recoveries between 97.6 to 99.0%, the relative standard deviation of 0.71 to 2.6%.

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