Effect of Xiaoaiping on the Growth and Angiogenesis of H22 Hepatic Carcinoma in Mice
|Keywords||XAP H22 liver cancer Vascular Endothelial Growth Factor Microvessel Density Angiogenesis|
Objective: Hepatocellular carcinoma (HCC) is common and one of the highest level of malignant tumor malignant throughout the world. Its incidence and mortality rank the f-orefront of cancer. It is a serious threat to people’s health and life. However, there had been no drug which has no little toxic or side-effect and good effect to cure the liver cancer up to now. Xiaoaiping (XAP) as a Chinese herbal drug is a sterilization on Marsdenia tenacissima hydrosolvent. The studying of pharmacology for Marsdenia tenacissi-ma has found that steroid glycoside and polysaccharrides which isolated from Marsdeniatenacissima, have an obvious effects against cancer. XAP may promote tumor cell diffe-rentiation or induce appoptosis of cancer cells to inhibit tumor growth in vitro and viv-o study. XAP are often used lonely or combined with radiotherapy, chemotherapy for v-ariety tumor patients in clinical practice, whereas its specific mechanism of anti-tumor were still not clear. HCC is rich of blood vessels. Angiogenesis plays an important rolein the process of tumor development. Therefore, the subject start with the tumor angio-genesis, through the effects of XAP different concentrations in tumor angiogenesis in vi-vo effects in mice tumor and discuss its possible mechanism in inhibition of hepatic ce-llular cancer growth in order to provide experimental evidence for clinical treatment of liver cancer.Methods: 75 female KM mice whose weight is about (20±2)g were injected H22 livertumor cells. They were divided into five groups: D1、D2、X1、X2、X3 and with 15 mice every group after three days they were injected. From the sixth day after injected,group D1 was peritoneal injected with 5-fluorouracil(5-Fu) as a positive control, group D2 was peritoneal injected with NS as a negative control and groups X1, X2 and X3 were peritoneal injected with XAP of 10g/Kg·d,20g/Kg·d and 40g/Kg·d. After 3 w-eek’s treatment, the mice were sacrificed and taking blood from eyeballs and the tissue of xenografted tumors were taken the next day. Measure the length and width of everyxenografted tumor then calculate the tumor’s volume and draw the tumor growth curve.Weight every xenografted tumor and calculate itsinhibitory rate. Get a part of the tumor(1×1×0.5cm) and put it in 10% formaldehyde solution fixed. Coloretur by hematoxylin and observe its pathological changes by light microscope. To detect the microvessel de- nsity (MVD) marked by CD34 in the xenografted tumor tissue by immunohistochemisty(IHC). The concentration of serum VEGF was measured by ELISA. Except the volume of the xenografted tumor was described by growth curves, the remaining measurement data were describe by((x|ˉ)±s ) and analyzed by statistical software with SPSS16.0 throughanalysis of variance. It is considered statistically significant when P≤0.05.Results: 1. The volume of the xenografted tumor were slower in the groups of low do-se and medium dose grpup of XAP. The volume of the xenografted tumor were the sl-owest in groups of 5-Fu and high dose group of XAP. The volume of the xenografted tumor were fastest in group NS. Compared with the group NS, the volume of the xen-ografted tumor were smaller in the treatment groups in each time point. The volume of the xenografted tumor were significantly smaller than the group NS especially in 21th in the treatment phase point.2. Compared with the group NS, there was a significantly difference in the average tu-mor weight of the treatment group. The inhibition rate in the groups of 5-Fu and high dose group of XAP were 51.2%, 49.6%. There was mo significantly difference between them. The inhibition rate in the groups of low and medium dose groups of XAP were 51.2%, 49.6%. There was a significantly difference between the two groups and 5-Fu.3. HE staining showed: There was more necrosis in the groups of 5-Fu and high dose group of XAP than in the group NS and there was some necrosis in the groups of lowdose and medium dose grpup of XAP.4. Compared with the group NS, there was a significantly difference in the dose of ser-um VEGF in the group of high dose group of XAP and so did the groups of low dos-e and medium dose grpup of XAP.5. Compared with the group NS, there was a significantly difference in the indexes su-ch as MVD in the group of high dose group of XAP and so did the groups of low d-ose and medium dose grpup of XAP.Conclusion: XAP of different doses can inhibit tumor growth of H22 liver cancer in mice and the high dose of XAP has the best inhibition. XAP may reduce VEGF in bl-ood and decrease MVD in tumor to make tumor ischemia and necrosis in order to inh-ibit tumorgrowth.