Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology

Effect and Mechanism of Asiatic Acid on Angiogenesis

Author JiangXuDong
Tutor LiuFang
School Huazhong University of Science and Technology
Course Hematology
Keywords asiatic acid Eahy926 angiogenesis tube formation apoptosis ERK1/2
CLC R285.5
Type Master's thesis
Year 2011
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Objective: To study the effect and the molecular mechanism of asiatic acid on angiogenesis in Eahy926.Methods: To study the effect of asiatic acid on angiogenesis,we used Annexin V and PI double staining,Hoechst33258 staining and two endothelial cell basal model:(1)migration,(2)tube formation using Eahy926, an endothelial cell line. To study the molecular mechanism of asiatic acid on angiogenesis, we used western blot to detect the level of ERK1/2, p-ERK1/2 , Akt, p-Akt.Results: Results demonstrate that certain concentrations of asiatic acid (25μmol/L、30μmol/L ) induces Eahy926 apoptosis, inhibits Eahy926 migration, inhibits tube fomation, We also found the level of ERK1/2, p-ERK1/2 is decreased by various concentrations (20μmol/L、25μmol/L、30μmol/L ) of asiatic acid in Eahy926.Conclusion:Asiatic acid inhibits Eahy926 migration and tube fomation. Asiatic acid suppresses tube formation in Eahy926 endothelial cell may be associated with Eahy926 apoptosis through inactivation of survival signal ERK1/2.

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