Mechanisms of EGCG Attenuating Focal Cerebral Ischemia and Reperfusion Injury in Rats
|School||Huazhong University of Science and Technology|
|Keywords||Table epigallocatechin gallate Cerebral ischemia -reperfusion injury Inflammatory cytokines Oxidative Stress Apoptosis|
[Objective] To establish the rat focal ischemia-reperfusion injury model, and to explore the protective effect and mechanism of epigallocatechin gallate catechin gallate (EGCG) on cerebral ischemia reperfusion injury. 【Method】 the suture method blocking rat right middle cerebral artery 2 hours after the removal of reperfusion, the preparation of rat focal ischemia-reperfusion injury model. 54 male SD rats, weighing 200 to 250 g, were randomly divided into sham operation group (sham group), ischemia-reperfusion injury group (I / R group) and EGCG group (E). Cerebral ischemia in rats injected with 0.1mg of EGCG to the right lateral ventricle after the start of the sham group and I / R group were given normal saline. 24 hours after reperfusion neurological dysfunction score Triazole 2,3,5 - triphenyltetrazolium chloride (TTC) staining method for the determination of infarct volume, ELISA assay of brain tissue cytokines TNF-α, IL-1β, spectrophotometer brain tissue MPO activity, SOD activity, thiobarbituric acid method for the determination of MDA content in brain tissue HE staining of pathological changes of brain tissue in situ terminal deoxynucleotidyl transferase labeling (TUNEL) method determination nerve neuronal apoptosis, immunohistochemical determination of Bcl-2, Bax. 【Results】 ischemia-reperfusion injury 24 hours, signs of neurological deficit in rats significantly increased cytokine TNF-α, IL-1β, increased apoptotic cells. Compared with the I / R group of EGCG group of nerve function defect improvement, reduced infarct volume, reduce the level of TNF-α, IL-1β, MPO activity decreased SOD activity increased and MDA decreased, histomorphological injury alleviate wither The decrease in apoptotic cells of Bcl-2 protein expression increased, Bax reduce. Discussion by alleviating oxidative stress and inflammatory responses, apoptosis and numerous studies have confirmed the occurrence and development of oxidative stress, inflammatory response, and apoptosis and cerebral ischemia-reperfusion injury, there are a lot of literature play a role in brain protection. The study found that cerebral ischemia reperfusion injury by EGCG treatment reduce the inflammatory response and oxidative stress, reduced apoptosis, so consider EGCG improve neurological deficits and pathological features and reduce infarct volume and brain protective effect may be related to the above mechanism . [Conclusion] EGCG can reduce the inflammatory cytokines TNF-α in rat focal ischemia reperfusion, the level of IL-1β and reduce oxidative stress, which can reduce brain tissue damage caused by ischemia and reperfusion play a cerebral protection role.