Transplant Human PBMC to Establish a "Human-mouse" Xenogeneic Graft-versus-host Disease Model
|School||Huazhong University of Science and Technology|
|Keywords||Organ transplant Xenograft Graft-versus- host disease|
Experiments due to the constraints of the moral, ethical, and legal aspects in the human body and is strictly limited to research involving human cells generally can only be carried out in vitro experiments, the results of in vitro experiments often there are some differences in the in vivo situation; \mouse model, provides the possibility for in vivo studies. However, the reported human - mouse model is also more effective and more stable method has rarely been reported. For reference xenogeneic graft-versus-host disease model (graft-versus-host disease, GVHD) \This study investigated choose the right mouse strains, optimization of the relevant experimental conditions, to establish a stable \The main content and the results are as follows: This study selected nude mice, NOD / SCID by sublethal doses of gamma-ray body irradiation, intraperitoneal infusion of healthy human peripheral blood mononuclear cells (PBMC) dissimilar acute X-GVHD model. Infiltration tail venous blood, tissue, organs and other indicators (through the detection of human T cells by flow cytometry and immunohistochemistry), the compared mouse model of human immune cells infiltration rate, survival time, thereby determining the establishment of a suitable X-GVHD model mouse strains, and fumble infusion pathway in human PBMC and a suitable amount of cells, and the best time window observed phenotypic changes and function of immune cells. The results showed that NOD / SCID mice is more suitable for the induction of \× 107 PBMC can successfully create a model of \Model using optimized experimental conditions, the dynamic changes of the monitoring of human T cell phenotype and function best time window of 7-11 days; mouse model, the average survival time was 14.16 ± 1.77 days. Innovation and significance of the study: In this study, using the different mouse model, post-test, to establish that the \\Meanwhile, the new model can be used for the selection and design of the in vivo susceptibility test prediction guide clinical treatment programs, as well as explore radiotherapy, endocrine therapy, immunotherapy, gene therapy and molecular targeted therapy treatment.