Dissertation > Medicine, health > Internal Medicine > Digestive and abdominal diseases > Pancreatic diseases

The Expression and Inflammatory Correlation of md2 in the Brain of Rats after Inducing Severe Acute Pancreatitis

Author MaXiaoYan
Tutor LiuJun
School Huazhong University of Science and Technology
Course Internal Medicine
Keywords pancreatitis blood brain barrier md2 TNF-α permeability
CLC R576
Type Master's thesis
Year 2011
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Objective: To explore the expression and inflammatory correlation of md2 in the pathphysiology of pancreatic encephalopathy during severe acute pancreatitis.Methods: 64SD rats were randomly divided into SAP group and Control group.SAP model was induced by retrograde bile-pancreatic duct infusion of 5% sodium taurocholate(NaTc).The control group was infused 0.9% Sodium chloride by the same methed. In all rats, the pancreatic pathology, the permeabilityof BBB, the expression levels of md2 and TNF-αin brain were observed at 2, 6, 12, 24h after operation.Results: Compared with the control groups, the pancreatic tissue in SAP groups presented a lot of edema ,hemorrhage,necrosis and inflammatory infiltrate cells.The expression level of md2 protein and md2mRNA showed remarkbly up-regulation in SAP groups(p<0.05).The level of TNF-αmRNA showed a correlation with md2mRNA at 2,6, 12,24h. The permeability of BBB showed remarkably increase at all time points (p<0.05).Conclusions: The SAP models were induced successfully by retrograde bilepancreatic duct infusion of 5% sodium taurocholate(NaTc). Md2 exists in normal cortex tissue and expresses remarkbly upregulation in the SAP groups. There were correlation between the permeability of BBB ,the expression levels of TNF-αmRNA and the expression levels of md2-mRNA .The express of md2 protein possibly participates in inflammatory signaling pathways of SAP, and leads to the increase of permeability of BBB and the release of inflammatory cytokins.

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