Dissertation > Medicine, health > Neurology and psychiatry > Neurology > Cerebrovascular disease

The Microcirculation Dysfunction and the Expression of Fgl2 in the Rat Model of Acute Cerebral Ischemia/reperfusion

Author ZhangPing
Tutor CaoFei
School Huazhong University of Science and Technology
Course Neurology
Keywords Acute cerebral ischemia-reperfusion injury Interleukin fiber Microcirculation In situ thrombosis
CLC R743
Type Master's thesis
Year 2011
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[Objective] To establish cerebral ischemia / reperfusion model of rat cerebral ischemia and necrosis , and to explore the possible mechanism of its micro- thrombosis . Taken detection of molecular biology methods in the rat brain tissue fgl2 expression to explore the correlation between the two , [Method] : The first part : take a total of 72 male SD rats . Randomly divided into sham operation group 36 , acute ischemia-reperfusion model group 36 , in which each group according to the time of reperfusion were randomly divided into one day group 12 , group 12 3 days , 7 days group 12 . 6 rats in each group were TTC staining infarction area . The remaining six separate brain frozen in liquid nitrogen , in part for HE staining and cellulose staining brain microvascular thrombosis , the other to take part in the ischemic area of brain tissue frozen in liquid nitrogen for use. Part II : Take frozen brain tissue was detected by immunohistochemical expression in brain tissue fgl2 , OK western blotting (Western Blot) fgl2 expression detected in brain tissue . [Results] The first part : 1.TTC staining of brain tissue after acute ischemia and reperfusion part of the brain tissue of no-reflow and necrosis. 2.HE staining of brain tissue seen in the model group cell disorder . 3 cellulose dyeing model group microvascular thrombosis see white sham group had no thrombosis. Part II: 1 . Immunohistochemistry showed that the model group rat brain capillary wall fgl2 visible expression , and in three days group was the most obvious. 2.western blot shows fgl2 model rats increased expression of brain tissue , and with three days group was the most obvious, and there is a positive correlation between the two . [Conclusion] rat brain tissue after acute ischemia and reperfusion , cerebral intravascular -situ thrombosis, there microcirculation in brain tissue , causing part of the brain tissue ischemia and hypoxia and necrosis. Increased expression of brain microvascular endothelial fgl2 involved in situ thrombosis.

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