The Expression of MiRNA Machinery Proteins in Different Types of Chronic Rhinosinusitis
|School||Huazhong University of Science and Technology|
|Course||Department of Otolaryngology - Head and Neck Surgery|
|Keywords||MiRNA machinery protein PACT Chronic rhinosinusitis Plasma|
Objective: To investigate the different expression of miRNA machinery proteins in the human nose of healthy individuals and patients with chronic rhinosinusitis without nasal polyps (CRSsNP),eosinophilic chronic rhinosinusitis with nasal polyps (Eos CRSwNP), and non-eosinophilic chronic rhinosinusitis without nasal polyps (Non-Eos CRSwNP). And to elucidate the significance of miRNA machinery proteins in the development of chronic rhinosinusitis .Method: The expression of miRNA machinery components (Dorsha、Dicer、PACT、TRBP、EIF2C2、FXR1) in human nasal biopsies was determined with real-time RT-PCR. Then immunohistochemistry and serial sections were used to futher vertify the presence of miRNA machinery proteins in human sinonasal mucosa.Results: The expression of Dorsha、Dicer、PACT、TRBP、EIF2C2、FXR1 was found in almost all specimens tested by means of real-time RT-PCR, but except for PACT, no differences were seen among the four groups. The mRNA level of PACT was significantly higher in patients with Eos CRSwNP and Non-Eos CRSwNP when compared with healthy individuals, and an elevated PACT level was seen in Eos CRSwNP when compared with CRSsNP . Immunohistochemical analysis revealed expression of PACT in biopsies of Eos CRSwNP and Non-Eos CRSwNP was obviously higher than that of normal sinonasal mucosa and CRSsNP, respectively. Immunohistochemical serial sections show presence of PACT in CD138+ plasma cells. Meanwhile, the tendency of CD138+ plasma cells in biopsies among the four gourps is consistent with the trend of PACT, and level of PACT is positively related with CD138, which has a strong significance in our experiment (r = 0.741, P = 0.000).Conclusion: The overexpression of miRNA machinery protein PACT in Eos CRSwNP and Non-Eos CRSwNP may imply an abnormal expression of miRNAs in CRS. PACT mainly expressed in cytoplasma of plasma cells, which suggests that regulatory mechanism of miRNA is involved in regulation of plasma’s function and leads to nasal polyps.