Dissertation > Medicine, health > Internal Medicine > Systemic disease > Poisoning and chemical damage > Alcohol poisoning

The Change of Pathology and Expression of Caspase-3 and CaN and Ion in Cerebral Cortex and Hippocampus and Cerebellum of Alcoholism Rats

Author JiaMingYue
Tutor ChenJiaFeng
School Jilin University
Course Clinical
Keywords Alcoholism Cerebrovascular diseases Apoptosis TUNEL caspase-3 CaN Ca2 Mg2 K ~ Na ~
CLC R595.6
Type Master's thesis
Year 2011
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Objective: With the improvement of people's living standards, the wine in the social and communicative activities is essential. Alcohol, alcoholism crowd has increased every year due to diseases caused by alcoholism is increasing year by year. Alcoholism has become a topic of common concern in the medical and social, to become a social threat. Long-term heavy drinking can cause multiple organ failure, alcoholism, damage to the central nervous system, the most serious complications. Serious impact on people's quality of life, increase the burden on society. However, very little about nervous system damage caused by the occurrence of chronic alcoholism machine Based on this, the experiment of chronic alcoholism apoptotic mechanism of occurrence and alcoholism after injury mechanism of hypoxic-ischemic encephalopathy research. Experimental methods: selection of 100 healthy male Wistar rats, mice aged 12-16 weeks, weighing 225-275g. Randomly divided into two groups, including alcoholism group 60, the brine control group 40. After one week of normal feeding started modeling. The alcoholism group: daily each rat of press 8ml/kg, 10ml/kg and 12ml/kg 50% alcohol gavage two weeks, one week, one week, daily gavage 2 times, intervals are six hours, a total of four weeks gavage, 50% alcohol anhydrous ethanol plus configured with distilled water. Body weights were measured weekly to observe the general biological characteristics; HE staining of pathological changes of the brain tissue; atomic immunohistochemical TUNEL staining neuronal apoptosis; immunohistochemical SP method for the determination of caspase-3 positive expression of CaN; absorption method for the determination of changes in the Ca2, K Mg2, Na and other trace elements. Experimental results: using disposable gavage mode, the model was successful; gavage one week after the start of alcoholic rats hair messy, dull, dry, gradually reduce food intake. Three weeks after hair removal, fatigue, weight loss, poor nutritional status and obviously, some appear cerebrovascular accident and nervous system damage performance, after 4 weeks, a significant difference in the weight of the two groups of rats exist; alcohol group with control rats simultaneously light microscope, HE staining, alcohol cerebral cortex, hippocampus, cerebellum, pyramidal cells decrease in the number of nerve cells disorganized, dispersed, some neuronal degeneration, necrosis; after TUNEL staining immunohistochemistry, alcohol group rat brain cortex, hippocampus, cerebellum apoptotic cells positive cell nuclei showed brown cytoplasmic homogeneous concentrated, deeply stained nuclei, a clear outline, small cell size, perinuclear crescent-shaped or annular edge set, and can be seen a few different sizes deep dyed brown small round shape of apoptotic bodies; immunohistochemical SP staining method for the determination of caspase-3 expression of CaN. Alcohol group and the control group were positive expression of caspase-3-positive cells showed brown staining cytoplasm presents, mainly located in the cerebral cortex, hippocampus and cerebellum, the distribution of parts and apoptotic cells were detected parts corresponding alcohol group compared to the control group, there was significant difference; two groups of rats CaN expression, alcohol group rat cerebral cortex, hippocampus, pyramidal cell coloring, clear boundary, located in the cytoplasm, the cell membrane visible protrusion was brownish yellow brown brown. The alcohol positive expression in cell number than the control group, a statistically significant difference. Less the cerebellum CaN positive expression, no statistically significant; the two groups of rats in vivo and in brain homogenates, atomic absorption method for the determination of trace elements, Ca2 content of the alcohol group than the normal control group increased significantly, Mg2, K Na content There was no significant change. Conclusion: Chronic alcoholism can cause cerebral cortex of the rat brain, the pathological changes in the hippocampus and cerebellum; chronic alcoholism can cause cerebral cortex, hippocampus and brain cell apoptosis, caused apoptosis corresponding parts of caspase-3 positive The expression; chronic alcoholism can cause cerebral cortex, hippocampus positive expression of CaN appear Ca2 content increases, the inference that the two may be involved in the development of alcoholism encephalopathy occurred.

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