Alpha-synuclein Expression in Brain after Alcoholism in Rats
|School||Henan University of Science and Technology|
|Keywords||Alcoholism Animal models α-Syn Neurodegenerative diseases Nerve damage|
Background and Purpose: α-Syn is the the presynaptic membrane expression of a soluble protein of the central nervous system. In recent years, foreign scholars in the study of alcohol addiction mechanism, α-Syn regulating the function of dopaminergic neurons, and dopaminergic system plays a critical role in the addictive poison (drug) material. This project through the establishment of acute, chronic alcoholism model, observed acute α-Syn expression in the chronic alcoholism rat brain tissue, to investigate the mechanism of the damage of alcohol on nerve cells, and to lay the foundation for further understanding of the physiological function of α-Syn. Methods: healthy and clean male SD rats (provided by the Experimental Animal Center of Henan University of Science and Technology) 42, were randomly divided into acute, chronic alcoholism, two groups of 12, in which chronic alcoholism group, the control group, chronic alcoholism group, n = 6; acute alcoholism group, the control group, acute 1h, 3h, 6h, 12h group, n = 6. Chronic alcoholism group at a concentration of 15% of the alcohol, 4 ml / d, 1 / d of the method The rats were gavage, serials 30d; acute alcoholism group at a concentration of 50% alcohol, 20g/kg manner right Rats were gavage. 2h after chronic alcoholism group gavage one month after the last feeding off myeloid rats were killed; of acute alcoholism Unit 1h model, 3h, 6h, 12h off myeloid rats were sacrificed. The experiment recorded during the rats body weight, temperament changes; anxious by HE staining, the pathological manifestations of the brain tissue of rats after chronic alcoholism; immunohistochemical staining observed acute, chronic alcoholism rat brain α- Syn expression data obtained using SPSS17.0 software t-test and analysis of variance. The results: (1) chronic alcoholism group Endoscopic view of visible the cerebral blood pipe Week gap widened subarachnoid leakage of bleeding, the reduced number of pyramidal cells, disorganized, and the cell gap widened Nissl body, nerve cells is unclear, visible nucleoli, chromatin margination. Purkinje cells decreased, local or even disappear; acute alcoholism group endoscopic view visible gap widened varying degrees of cerebral blood tube weeks subarachnoid leakage of bleeding, the part of the brain parenchyma vascular congestion, gliosis, arranged irregular, cerebellar congestion performance. ② chronic alcoholism rats brain cortex, brain stem α-Syn positive cells number increased in varying degrees; Acute Alcoholism model, with the extension of the time after the poisoning, rat brain cortex α-Syn-positive The number of cells peak at 6h 6h declined slightly, but the value is still higher than the acute 1h, 3h. ③ chronic alcoholism rat cerebral cortex, brain stem α-Syn average optical density were increased to varying degrees, the trends mean the same positive cells; Acute Alcoholism model, along with the time extension after the poisoning, groups large The average optical density of the rat brain cortex α-Syn increased to varying degrees, the trend with the number of positive cells. Conclusion: ① the rats acute and chronic alcoholism, the body's reaction, reduced exercise capacity, brain tissue pathological manifestations of cerebral blood tube week gap widened, the decrease in nerve cells, partially visible subarachnoid leakage bleeding; ② chronic alcoholism α-Syn positive cells in rat brain tissue mean, mean optical density of an upward trend; ③ acute alcoholism group, with the extension of the time after the poisoning, the α-Syn-positive cells in the brain tissue of rats mean average light density values ??peaked at 6h 6h declined slightly, but the value is still higher than the acute 1h, 3h.