Dissertation > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Bone marrow tumor

PGC-1α expression in multiple myeloma and myeloma associated with angiogenesis Relationship

Author ZhaoJiKai
Tutor GuoTao
School Huazhong University of Science and Technology
Course Department of Hematology
Keywords PGC-1α Angiogenesis Myeloma
CLC R733.3
Type Master's thesis
Year 2011
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Objective : PGC-1α is a gene involved in regulating the transcriptional activity of the important coactivator , studies have shown : its inducible expression , can promote angiogenesis , and does not depend on the classic hypoxia-inducible factor (HIF) pathway , but associated with estrogen receptor (ERRa) combined thus contributing to regulate the expression of VEGF angiogenesis . We will study the PGC-1α in multiple myeloma expression , and to analyze its related regulatory pathways in myeloma angiogenesis . Methods: Immunohistochemical labeling method initially detected PGC-1α protein in the bone marrow biopsy and tumor cell lines in the expression and distribution ; using RT-PCR was used to detect PGC-1α and its related regulatory pathways in the expression of each factor ; using immune histochemical markers of bone marrow microvessel analysis myeloma bone marrow microvessel density and distribution of PGC-1α expression correlation . Results: The study found that PGC-1α in the blood system diseases , only highly expressed in MM, I have less than or equal to the normal median level , PGC-1α ongoing myeloma patients with high expression in the cytoplasm of tumor cells into , but its myeloma cell line RPMI8226 and U226 are mainly located in the nucleus , in PGC-1α expression in patients with high bone marrow microvessel density higher . Involving PGC-1α expression regulatory pathways underlying factor analysis showed that : MEF2C, ERRα, PPARγ were expressed at the RNA level . In addition , PGC-1β- and PGC-1α homologous another PGC-1 family members in the blood of tumor protein level is relatively broad , and its positioning in a variety of cell nucleus or cytoplasm . Conclusion : PGC-1α and its associated regulatory pathway may be involved in myeloma and its associated development and progression of angiogenesis .

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