Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology

Noble Dendrobium Polysaccharides Attenuate Learning and Memory Deficits Induced by Lipopolysaccharide and Its Mechanisms in Rats

Author WangLiNa
Tutor ShiJingShan
School Zunyi Medical College,
Course Pharmacology
Keywords Noble dendrobium polysaccharides lipopolysaccharide learning memory IL-1β TNF-α TGF-β1 iNOS COX-2
CLC R285.5
Type Master's thesis
Year 2011
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Objective:To investigate the protective effect of Noble dendrobium polysaccharides (NDP) on lipopolysaccharide (LPS)-induced learning and memory deficits in rats, and explore the active mechanisms.Methods:Treated with NDP (40,80,160mg·kg-1·d-1) for 7 days, the rat model with learning and memory deficits was induced by injection of LPS 20μg (2μg·μl-1) into the lateral ventricle. The abilities of spatial learning and memory of the rats were tested by Morris water maze. The mRNA levels of TNF-a, IL-1β, TGF-β1, COX-2 and iNOS were detected by real time RT-PCR, and the expressions of TNF-a, IL-1β, TGF-β1, COX-2 and iNOS proteins were examined by Western Blotting.Results:Compared to the sham-treated rats, LPS injection significantly prolonged the mean escape latency in the navigation test and shortened the adjusted escape latency, and the mRNA and protein expressions of TNF-a, IL-1β, TGF-β1, COX-2 and iNOS in hippocampus of the rats were notably increased. However, NDP treatments significantly decreased the mRNA and proteins expressions of TNF-α, IL-1β, TGF-β1, COX-2 and iNOS.Conclusion:NDP can attenuate the ability of spatial learning and memory deficits induced by LPS, which may be due to decreases of the mRNA and protein expressions of TNF-α、IL-1β、TGF-β1、iNOS and COX-2.

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