Dissertation
Dissertation > Medicine, health > Internal Medicine > Heart, blood vessels ( circulatory ) disease > Abnormal blood pressure > Hypertension

Effect of Grape Seed Procyanidins on the Blood Pressure in Renovascular Hypertensive Rats and Its Mechanism

Author GuoRongNian
Tutor WuXiuXiang
School Zunyi Medical College,
Course Pathology and Pathophysiology
Keywords grape seed procyanidins renovascular hypertensive rats eNOS ET-1 SOD MDA TNF-α IL-10
CLC R544.1
Type Master's thesis
Year 2011
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Objective:To investigate the effect of grape seed procyanidins (GSP) on the blood pressure in renovascular hypertensive (RH) rats and its mechanism.Methods:2K1C method was used to establish the RH rat models. Meanwhile,8 rats were served as controls. After 2 weeks of operation,32 rats which tail systolic pressure increased above 130 mmHg were selected as successful RH rats, and divided into 4 groups (n=8) randomly:renovascular hypertensive model (RH model); low GSP treated (low GSP,50 mg·kg-1·d-1); high GSP treated (high GSP,200mg·kg-1·d-1) and captopril treated group (captopril,30 mg·kg-1·d-1). Tail systolic pressure was measured at the same time weekly. The activity of superoxide dismutase (SOD), malonaldehyde (MDA) and nitric oxide (NO) levels and total antioxidant capacity (T-AOC) in serum were determined respectively 6 weeks after treatment. ELISA was performed to detect the TNF-a and IL-10 levels in serum. Western blotting was used to evaluate the expressions of endothelial nitric oxide synthase (eNOS) and endothlin-1 (ET-1) in abdominal aortic tissues.Results:Compared with control group, the tail systolic pressure in RH model group was increased significantly 6 weeks after GSP treatment. In the RH model rats, treatment with GSP reduced the tail systolic pressure, MDA and TNF-a levels in serum and the expressions of ET-1 in abdominal aortic tissues, increased the activity of SOD, IL-10, NO levels and T-AOC in serum, as well as the expressions of eNOS in abdominal aortic tissues.Conclusion:GSP can decrease tail systolic pressure significantly of RH rats by increasing the antioxidative ability, the expressions of eNOS and NO production in vascular endothelial cells, inhibiting inflammatory mediator TNF-αand the expressions of ET-1, and increasing the level of anti-inflammatory mediator IL-10.

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