Antitumor Effects of Elemene on the Proliferation Inhibition and Apoptosis Induction in Ascites Hepatoma Cell Line H22
|School||Dalian Medical University|
|Course||Traditional Chinese Medicine|
|Keywords||H22 liver cancer Mice Elemene Proliferation Apoptosis|
Objective: To investigate the antitumor effects of Elemene on the proliferation inhibition and apoptosis induction in ascites hepatoma cell line H22.Methods: 35 healthy adult Balb/C rats were randomly divided into 5 groups: high dose of Elemene group （100 mg/kg）, low dose of Elemene group （50 mg/kg）, Elemene （50 mg/kg） combined with 5-FU （20 mg/kg） group, 5-FU group （20 mg/kg）, control group （0.9%NACL）. Mouse models of the solid tumor of liver cancer were established. The inhibitory rates of solid tumor were observed. The rates of cell viability were observed by MTT. Apoptotic cells were detected by AnnexinV-FITC/PI staining and TUNEL Labeling methods..Results: The rates of tumor-inhibitory were different in various drug groups, as 5-FU group showed the most significant effect. Between the two groups, the high-dose of Elemene group and low dose of Elemene group, we observed the volume of tumor in high-dose of Elemene group was small. In the first day after treatment, there was no significant difference between the drug groups and control group on tumor size. In the next 3 days, 5-FU group and combination group were significantly different from control group （*P <0.05）. In the coming days, the volumes of tumor in the 4 drug groups were statistically different from control group （* P <0.05, **P <0.01）. After H22 cells were treated with various concentrations of drug groups, cells growth were significantly inhibited in a dose and reaction time dependent manner. Apoptosis occurred in the early stage was identified by AnnexinV-FITC/PI staining and apoptosis were also detected by TUNEL Labeling methods. The high dose Element group has more effective apoptosis induction than the other groups. Elemene could obviously inhibit cell growth in H22 cells, which through induce the occurrence of apoptosis. For the internal mechanism of apoptosis still needs further research.