The Experimental Study of Semiconductor Laser Radiation and 5-FU Sustained Release Repressed Oral Malignant Tumor Cell
|School||Anhui Medical University,|
|Keywords||antiagiogenic inhibitor human Oral squamous cell carcinoma cancer semiconductor laser KB cell ACC-3 5-fluorouracil cell toxicity factor cytoskeleton cell cerato-molecule anti-tumor activity intermediate type filament fusion protein sustained release proliferation|
Oral malignant tumor is one of the most common tumors in the world, 5% of the whole body malignant tumor, in which 90% is the squamous cell carcinoma from epithelium. In recently, the outbreak rate of the oral malignant tumor is present up-trend, the patient age also trends to younger. As result, investigating the pathogenesis of oral malignant tumor and then to look for actively and utility prevention path have been a very urgent important mission. Some scholars think that one of the materiality tumor’s two main characteristics is a great deal of new-born blood vessel forms. The malignant tumor cells get necessary nutrient from new-born blood vessel organization, taking a great deal of metabolism, in the same time, the tumor cells pass the new-born blood capillary by blood to transfer. The vessel forms from already exist blood capillary and the growth of blood capillary post veinule’s new blood capillary vessel. The new-born vessel have important meaning for the growth, graduation and transfer of tumor. The tumor could grown malignantly and transfer successfully after new-born blood vessel phenotype appeared.The formation of vessel is also the key factor that decides the oral malignant tumor’s growth, differentiation, invasion and transfers.The consequence demonstrated that the two groups of the tumor cell population in PCNA and Histone both decreased, the reduce showed that the cellular proliferation of KB cell and ACC-3 cell strains were all restrained in two groups. The exposure time which the biggest influence toKB cell and ACC-3 was 90seconds and 120seconds separately (energy density was 5.58J/cm2 and 7.8J/cm2 )。Under such dose radiation, the decrease rate of theKB cell and ACC-3 tumor cell strains were 76% and 66% respectively. Except that, the integrity of microtubule and intermedius type structure of these cells in two groups tests were also destroyed. The Plectin and Synemin of intermedius type’s GAP-associated protein GAP decreased too.After daily injection (i.p.) for 12 days, the tumor growth in the treated group was only one-third the size as compared with that of the control group. The growth rate in the treated group is also significantly slower than that of the control group. This decrease in tumor size was consistent with decrease in CD31-positive vasculature. We hope that this novel bifunctional protein will contribute significantly to human Oral squamous cell carcinoma cancer therapy.Our study’s some significant findings were on below: semiconductor laser radiation and 5-Fu sustained release would repressed KB cell and ACC-3 tumor cells’s suppressing effect, which showed two directions:(1)repressed cell caryocinesia, which made cell proliferation stop and decrease to lead to decrease the cell population. Semiconductor laser radiation and 5-Fu restrained the produce of Histone, which made DNA replication unably to result in proliferation of KB cell and ACC-3 tumor cells decreased;(2)Semiconductor laser and Tca8113 accelerated the cell death by effected cell metabolism or structure. This mechanism of depressant effect may be that microtubule and cell cerato-molecule structure changed and losed cell suport function for Plectin and Synemin. This provided related rationale for clinical oral malignant tumor treat.