Changes and Significance of the Tissue Factor Pathway Inhibitor and Thrombomdorlin Following Acute Pulmonary Embolism in Rabbit
|School||Hebei Medical University|
|Keywords||pulmonary embolism TFPI - 1 TM clotting pathway rabbit|
Objective:Anticoagulation system and coagulation system are constantly changing in the acute pulmonary embolism occurrence and development process. As the core of the thrombotic diseases-clotting mechanism, it need research to understanding of the disease accurately. Tissue factor is the important starting factor of exogenous clotting way, it’s join the coagulation process first to start the process,and the tissue factor pathway inhibitor play an important role by Interact with the tissue factor. Thrombomdorlin also join some phase of the coagulation process to control clotting. The tissue factor pathway inhibitor and thrombomdorlin play an important role in the coagulation process.The test detect the change of the TFPI-1 and TM in plasma by Establish rabbit pulmonary embolism model to explore the TFPI - 1 and TM changing trends and their respective anticlotting pathway on behalf of the significance in the anticoagulant process.Method:sixty-four New Zealand rabbits, weighing 2.5～3.5kg, the male and female were given consideration to, were obstructed the left lower lung artery by inflating gas of 5F Berman sacculus catheter to set up rabbit APE model. The animals were randomly divided into eight groups: (1) control group(n=8), (2)sham operation group(n=8), (3)embolism 1h group(n=8), (4)embolism 2h group(n=8), (5)embolism 4h group(n=8), (6)embolism 8h group(n=8), (7)embolism 12h group(n=8), (8)embolism 24h group(n=8). Using 5F needles Piercing from right neck venipuncture after the experimental animals anesthesia. The 6F artery sheath pipe is put along the guidewire. Making catheter in turn through the rabbit catheter superior vena cava, the right atrium, right ventricular, pulmonary artery, finally achieved left-down pulmonary artery under X-ray. We monitored the pulmonary arterial mean pressure (PAMP) at pre-embolization, embolization instantly, embolization1h, 2h, 4h, 8h, 12h and 24h. We detected the content of TFPI-1 and TM in plasma by ELISA method at embolization1h, 2h, 4h, 8h, 12h and 24h.Result:1 general condition: the heart rate and breath of sham operation group and control group are steady After anesthesia and surgery in the process. the heart rate and breath of the embolismation group is accelerate when the catheter block the Left-down pulmonary artery.2 hemodynamic changes: Except the sham operation group, the PAMP of the Embolism instantly rise sharply,is higher than the sham operation group and control group(P<0.05). the PAMP of the embolismation 0.5h start to decline, Embolismation 1h still decline.But the PAMP of the embolismation 0.5h, 1h is still higher than the control group(P<0.05). The PAMP of the embolismation2h,4h,8h decline to the sham operation group and control group’s level(P>0.05).The PAMP of the embolismation 12h, 24h rise again and is higher than the sham operation group and control group obviously(P<0.05). The PAMP of the sham operation group isn’t different from the control group and each embolismation group in the same time (P>0.05).3 The changes of the TFPI - 1 in Plasma: The TFPI-1 in the group of embolismation 1h, 2h is not different form control group(P<0.05) . The TFPI-1 in the group of the embolismation 4h,8h,12h,24h is higher than control group (P<0.05). The content of the embolismation 24h is the highest. The content of the sham operation group is lower than control group(P<0.05).4 The changes of the TM in Plasma:The content of the TM in control group is lower than the sham operation group, embolismation 1h,2h,4h,8h,12h,24h. The content of the TM in embolismation 12h start to decline. The content of embolismation 24h is higher than control group,is same to embolismation 2h. Conclusion:1 Pulmonary embolism led to pulmonary artery pressure, pulmonary circulation pressure increases.2 TFPI-1and TM participate in the happening and development of the pulmonary embolism through their own anticoagulant mechanism3 In the different stages of pulmonary embolism, the effect of the tissue factor pathway inhibitor pathway and protein C system is different. TM have the Strongest role in the early stage of the Pulmonary embolism. The role of the TFPI-1 strengthened Gradually in the later stage of the Pulmonary embolism.