Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology

Study on Pharmacological Effects of Fermentation Products from Medicinal Fungi Phellinus Igniarius

Author MengQingLong
Tutor WangZuo
School Jilin Agricultural University
Course Of Pharmacy
Keywords Phellinus Polysaccharide Fermentation products Pharmacological effects
CLC R285
Type Master's thesis
Year 2011
Downloads 171
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The mulberry of Huang [Phellinus igniarius, (Linnearus: Fries) Quelet] is a perennial the rare medicinal fungi, due to its unique pharmacological activity, become the hot research scholars at home and abroad, and known as the internationally recognized anti-cancer one of the good medicinal fungi. Traditionally the active ingredient is usually extracted from the fruiting bodies of Phellinus, but resources are scarce due to their wild Phellinus difficult artificial cultivation, which has seriously hampered the development and use of Phellinus in the field of medicine. The Phellinus submerged fermentation culture, can reduce cycle time and cost savings, reduced pollution, Get Phellinus active ingredient. In this study, obtained by submerged fermentation fermentation products, and its inhibitory effect, anti-inflammatory effects and anti-aging effect and attenuated synergies depth and meticulous research, for Phellinus extracellular polysaccharide capsule preparation process as well as anti- The tumor and immune-enhancing role of the System. The experimental results are as follows: (1) In this study, the antibacterial activity of different solvent extracts of Phellinus submerged fermentation product mycelium and fermentation broth, and Phellinus fermentation product inhibitory effect by heat treatment operation the thermal stability was discussed. The results showed that The Phellinus different extracts of mycelium and fermentation broth against Staphylococcus aureus (Staphylococcus aureus), Salmonella enteritidis (Salmonellae enteritidis), E. coli (Escherichia coli), Salmonella typhimurium (Salmonella typhimurium) 4 instructions Inhibitory: the mycelium methanol, n-butanol extract and fermented liquid indicator bacteria showed strong inhibitory effect, the minimum inhibitory concentration of The 0.1563 ~ 0.625mg/mL has a broad-spectrum of antibacterial activity, and dramatic changes in Staphylococcus aureus antibacterial activity with increasing temperature, showing good thermal stability, infer the presence of parts of the mycelium of Phellinus antibacterial substances body methanol, n-butanol extract and fermentation broth; At the same time, the mycelium butanol extract of the four kinds of indicator bacteria highest inhibitory rate, quality and minimum inhibitory concentration of only 0.1563 ~ 0.3125mg/mL , which said the yellow Mingsang mycelium butanol extract of the strongest antibacterial activity. (2) In this study, igniarius fermentation product of intracellular polysaccharide content of PIP and PIE were determined, and xylene-induced mouse ear edema, carrageenan-induced paw edema in mice with acute inflammation and cotton balls induced chronic granulomatous inflammation of the mice as a model to study its anti-inflammatory effects. Experimental results show that the the Phellinus intracellular, extracellular polysaccharide PIP and PIE xylene-induced ear edema in mice the highest inhibition rate were 41.68% and 47.01%. Chronic inflammation model of carrageenan-induced paw edema in mice model of acute inflammation and cotton balls induced granuloma had some inhibitory effect, and they can enhance the spleen index and thymus index, that mice own immune system has improved to some extent. PIP and PIE shows some differences in the anti-inflammatory effect may be related to intracellular, extracellular polysaccharide structure related to the different. 3 In this experiment, sarcoma S180 tumor bearing mice as experimental models of intracellular fermentation product, Phellinus polysaccharide PIP and PIE anti-tumor effect of chemotherapy drugs attenuated synergies were studied. Experimental results show that the the Phellinus intracellular, extracellular polysaccharide PIP and PIE against chemotherapy drug-induced body weight loss, abnormal peripheral blood and immune organs such as spleen and thymus atrophy toxicity, and can effectively improve the anti-cancer effect of CTX, CTX United Phellinus intracellular polysaccharide PIP and PIE highest inhibition rates were 64.84% and 68.96%, respectively. Phellinus intracellular polysaccharide PIP and PIE Antitumor immunity enhancement and to improve the ability of IFN-gamma activity, which may be one of the mechanisms of the attenuated efficiency In this experiment, materials selection, particle flow, particle bulk density, particle moisture percentage as index Phellinus extracellular polysaccharide capsule preparation molding process. The results showed that the percentage of moisture determination of the different powder mixed program, and ultimately select the corn starch as the Phellinus extracellular polysaccharide capsule accessories. Optional granulation was carried out, the effect is preferably 90% ethanol, and dried under reduced pressure at 60 ° C, whole. The average bulk density of the particles was determined 0.553g/mL so the selection of No. 0 capsules, and adjust the amount of sample loading so that each piece as 0.4 g. The determination of the particles have an average angle of repose of 32.794 °, its good fluidity, easy packing. Determination of particulate critical relative humidity of about 60%, Therefore, in the the Phellinus extracellular polysaccharide capsule production, packaging and storage environment, the relative humidity must be controlled below 60% in order to reduce the impact of moisture on the stability of the particles, so as to ensure the smooth progress of production, packaging and storage, and the stability of the finished product. 5 the experimental the sarcoma S180 mice H22 liver cancer mice as experimental model, anti-tumor and immune-enhancing and life-prolonging effect of Phellinus extracellular polysaccharide capsule, and its toxic effects were investigated. The results show that the Phellinus extracellular polysaccharide capsule on mice sarcoma S180 and H22 liver cancer have a better inhibition significantly reduced tumor weight inhibition rate improved significantly. In addition, the Phellinus extracellular polysaccharide capsule play a role in cancer while also effectively improve the immunity of sarcoma S180 and H22 liver cancer in mice, and during the administration did not inhibit the increase in body weight of mice, the thymus index and spleen index, and weight have varying degrees of improvement. Phellinus extracellular polysaccharide capsule are to some extent effectively extend the survival time of sarcoma S180 and H22 liver cancer in mice, mice in which the sarcoma S180 low-dose group life extension rate of 23.97%; H22 liver cancer high-dose group life extend the rate of 53.86%. Acute toxicity test measured Phellinus extracellular polysaccharide capsule is an actual non-toxic substances, has laid a solid theoretical foundation for the development of high efficiency and low toxicity anti-tumor drugs. 6 In this experiment, D-galactose induced subacute aging mice experimental model, serum, brain, liver tissue superoxide dismutase (SOD), glutathione peroxidase (GSH-PX ), catalase (CAT) activity and malondialdehyde (MDA), Determination of change, anti-aging effect on the of Phellinus extracellular polysaccharide PIE. The results showed that Phellinus the extracellular polysaccharide PIE, in the low dose group and model group, can significantly increase the serum, liver, brain tissue SOD and GSH-PX and CAT activity, and reduce the content of MDA, indicating that The Phellinus extracellular polysaccharide only enhance the body's defense mechanisms of free radical damage, and can effectively reduce the damage of lipid peroxides tissues or cells, further reveal the Phellinus extracellular polysaccharide can play an anti-aging effect by improving the metabolism of free radicals and the ability to antagonize free radical damage due to the aging process of aging mice. In summary, Phellinus extracellular polysaccharides have anti-aging effects, the specific mechanism may be related to scavenging oxygen free radicals and reactive oxygen species, as well as enhance the body's antioxidant enzyme activities. This innovation: This article Phellinus intracellular, extracellular polysaccharide anti-inflammatory effect for the first time a systematic study; first time Phellinus intracellular, extracellular polysaccharide to chemotherapeutic drugs attenuated synergies research; initial igniarius Preparation of a preliminary study of extracellular polysaccharide capsule, and its anti-tumor effect.

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