MIF, TNF-α in hepatocellular carcinoma and clinical significance
|School||Anhui Medical University,|
|Keywords||HCC MIF TNF-α Tumor suppressor gene Targeted therapy|
Objective: To investigate the MIF and TNF-α protein and liver cancer, development and the relationship between clinical characteristics. Expect from molecular biology to explore the feasibility of targeted therapy of liver cancer. Methods: First Affiliated Hospital of Anhui Medical University Department of Pathology and surgical resection specimens were pathologically confirmed 60 cases of liver cancer as the experimental group, all patients did not receive preoperative chemotherapy, radiation or other cancer treatment, and a complete follow-up data. Another 20 cases of corresponding adjacent normal tissue as control group. Antibiotic streptomycin using a biotinylated protein (SP) method immunohistochemistry liver tissue, adjacent normal tissue MIF and TNF-α protein levels; analyze these indicators and liver size, gross type, depth of invasion, tissue differentiation and clinical stages; and the relationship between these indicators. Results: 60 cases of hepatocellular carcinoma, MIF protein expression in 46 cases positive, the positive rate was 76.6% (46/60); TNF-α protein expression in 12 cases positive, the positive rate was 20% (12/60 ). In 20 cases of adjacent normal tissues, MIF and TNF-α protein expression was positive in 6 patients (30.0%) and 16 cases (80.0%). Cancer tissues showed high expression of MIF protein, TNF-α protein expression was low, and the MIF protein in cancer tissue was significantly higher than in adjacent normal tissue, on the contrary cancer tissue TNF-α protein expression was significantly lower than in adjacent normal tissue , showed significant differences (p lt; 0.05). Tip HCC MIF and TNF-α protein expression, affecting the normal protein expression levels, suggesting that MIF pass through certain signaling pathways to promote tumor development and metastasis, TNF-α may induce signal transduction pathway through cytokines, maintain procedures for normal tissue cells apoptosis, thereby inhibiting the growth of tumor cells, the expression levels of both the occurrence and development of liver disorders play a role, both in the process of tumor development there might be some correlation. However, the signal transduction mechanism for domestic and foreign experts have not been studied thoroughly, specifically related mechanism needs further study. Conclusions: (1) adjacent normal tissues MIF expression was significantly lower than HCC, contrary TNF-α in adjacent normal tissue expression was significantly higher than in HCC tissues, MIF-induced signaling pathway may play in the formation of liver cancer In an important role, TNF-α induced signaling pathway may inhibit the pathogenesis of HCC. (2) liver cancer high expression of MIF and TNF-α protein low expression, and in some way to promote and inhibit the occurrence of liver cancer development and metastasis, suggesting that blocking MIF and activation and TNF-α-induced signal transduction pathway may In the development of liver cancer prevention play an important role in providing targeted therapy for liver cancer molecular basis.