Dissertation
Dissertation > Culture, science,education, sports > Sports > Sports theory > Fundamental Physical Science > Exercise Physiology

Endurance Exercise, Resistance Exercise on the Aging Mouse Endoplasmic Reticulum Stress-mediated Apoptosis in Skeletal Muscle Cells Intervention

Author LiHaiFeng
Tutor LuJian
School East China Normal University
Course Human Movement Science
Keywords Sarcopenia endurance exercise resistance exercise gene expression GADD153/CHOP ASK1/JNK Caspase-12
CLC G804.2
Type Master's thesis
Year 2011
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At present, population aging has become a global countries and regions need to face and solve problems. Aging of the population in all aspects of human life will have a profound impact, in which older individuals on health care and nursing care intensity is particularly prominent. How to make older individuals enjoy a high quality standard of living has become a close attention to all areas and to address the issue. Tufts University Professor Irwin Rosenberg in 1988, the concept put forward Sarcopenia, defined as a place with aging and degenerative pathology of skeletal muscle, manifested as skeletal muscle mass, volume, and decreased muscle strength.The results show that the parties in recent years, Sarcopenia the occurrence and development is closely related with cell apoptosis, are known to have three major-mediated apoptosis signaling pathways:mitochondrial-mediated apoptosis signaling pathways; death receptor factor Mediated apoptosis signaling pathways and endoplasmic reticulum stress-mediated apoptosis signaling pathways. Mitochondrial damage pathway (endogenous pathway) and death receptor activation pathway (extrinsic pathway) is mediated by the two classical pathway of apoptosis, endoplasmic reticulum stress pathway of apoptosis activated a new cell Apoptosis pathway. Therefore, the endoplasmic reticulum stress-mediated apoptosis signaling pathways in the development process in Sarcopenia clarify the role and influence around the exercise of its will help us to understand with Sarcopenia mechanism of apoptosis occurred, to the adoption of the development Exercise intervention to prevent or delay the Sarcopenia way to the impact of the elderly.This long-term and short-term endurance by observing the movement and resistance exercise on quadriceps muscle cross-sectional area of C57 mice and the quadriceps muscle in GADD153/CHOP, ASK1/JNK and Caspase-12 mRNA expression to of different sports internal ER stress-mediated apoptosis in rats. At the same time for the selection of slow skeletal muscle attenuation of the exercise program provides a theoretical basis. Thislong-term and short-term endurance by observing the movement and resistance exercise on quadriceps muscle cross-sectional area of C57 mice and the quadriceps muscle in GADD153/CHOP, ASK1/JNK and Caspase-12 mRNA expression to Of different sports internal ER stress-mediated apoptosis in rats. At the same time for the selection of slow skeletal muscle attenuation of the exercise program provides a theoretical basis.Experimental Methods:This study selected 28 male C57 mice were used as subjects were randomly divided into life-long endurance exercise group (LE) n=8, life-long resistance exercise group (LR) n=8, aged endurance exercise group (OE) n=4, Age resistance exercise group (OR) n=4 and older sedentary control group (OC) n=4. The sample used for endurance exercise treadmill model, weight ladder as a resistance exercise model. Life-long training exercise group was 19 months old in the exercise group trained for 4 months, the quiet control group did not participate in training. After the training process in mice by HE staining changes in the quadriceps, and detected by RT-PCR instrument in mice quadriceps GADD153/CHOP, ASK1/JNK and Caspase-12 mRNA expression, and the data analyzed.Experimental results:1, After light microscopy, compared to samples of each group cross-section of quadriceps muscle fiber density and found that life-long resistance exercise group and old skeletal muscle resistance exercise group configuration compared with the other groups remain the most intact Compared with the aged control group, life-long endurance exercise endurance exercise group and the older group aged quadriceps cross-section is greater than the control group, and the distribution of muscle fibers compared with the aged control group and more compact.2, In the exercise group and the aged control group quadriceps GADD153/CHOP mRNA relative expression in comparison, the aged control group GADD153/CHOP mRNA relative expression in the quadriceps was significantly higher than the exercise group, and there was significant Differences (P<0.05). Besides lifelong endurance exercise group, the elderly and the elderly endurance exercise group compared with resistance exercise group had no significant difference (P> 0.05). The experimental group of life-long endurance exercise GADD153/CHOP mRNA was the lowest, compared with the other sports were significant differences (P<0.05).3, Compared with the aged control group, endurance exercise and resistance exercise can effectively reduce the mice within the ASK1/JNK mRNA expression in the quadriceps, and significant, indicating that endurance exercise and resistance exercise can be effectively inhibit ASK1/JNK mRNA expression, but comparison of results, resistance exercise ASK1/JNK mRNA expression inhibited the effect even more significant, indicating that resistance exercise in skeletal muscle aging in terms of significant resistance.4, Compared with the aged control group, endurance exercise and resistance exercise can be effective in reducing mouse shares four of the Caspase-12 mRNA expression, and significant. Tips endurance exercise and resistance exercise in skeletal muscle can be effectively inhibit the expression of Caspase-12 mRNA, but by the movement of life, the longer the campaign period, on skeletal muscle Caspase-12 mRNA Expression by the more apparent.Conclusion:1, HE staining results suggest that skeletal muscle in the fight against aging process, slow skeletal muscle resistance exercise is the preferred movement pattern of aging. Although endurance exercise has to a certain extent, the occurrence of delayed Sarcopenia, endurance exercise intervention was as effective as resistance exercise. The exercise group’s findings have been made against the effect of attenuation of skeletal muscle, indicating the intended movement pattern of the mouse cell structure is influential.2, Compared with the aged control group, endurance exercise and resistance exercise can be made within the GADD153/CHOP mRNA expression in mouse quadriceps muscle was significantly reduced, suggesting that endurance exercise and resistance exercise can be suppressed within the GADD153/CHOP mRNA expression in skeletal muscle And the impact of life without movement.3, In the exercise group and the aged control group quadriceps ASK1/JNK mRNA relative expression in comparison, the aged control group ASK1/JNK mRNA relative expression in the quadriceps was significantly higher than the exercise group, and there was significant Differences (P<0.05). Besides lifelong resistance exercise group, the elderly resistance exercise group ASK1/JNK mRNA expression of life than endurance exercise endurance exercise group and the elderly were lower, and there were significant differences (P<0.05). Life-long resistance exercise group, age resistance exercise group ASK1/JNK mRNA expression was significantly different (P <0.05), life-long endurance exercise endurance exercise group and the older group ASK1/JNK mRNA expression was no significant difference (P>0.05).4. Compared with the aged control group, endurance exercise and resistance exercise can be effective in reducing mouse shares four of the Caspase-12 mRNA expression, and significant. Tips endurance exercise and resistance exercise in skeletal muscle can be effectively inhibit the expression of Caspase-12 mRNA, but by the movement of life, the longer the campaign period, on skeletal muscle Caspase-12 mRNA Expression by the more apparent.

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