Effects of Tanreqing Injection on Acute Lung Injury Induced by Endotoxin in Rats
|School||Zhejiang University of Traditional Chinese Medicine|
|Course||Integrative Medicine Clinical Internal Medicine|
|Keywords||Tanreqing injection Acute lung injury NF-κB p65 Oxidation Antioxidant|
Objective: This experimental rat tail vein injection of LPS (lipopolysaccharide), to establish a rat model of acute lung injury ALI (acute lung injury), ALI rat lung tissue morphology the study Tanreqing injection, bronchoalveolar lavage fluid ( bronchoalveolar lavage fluid, BALF) polymorphonuclear neutrophils (polymorphonuclear neutrophils, PMN), lung tissue oxidation antioxidant factor nuclear factor-κB (NF-κB) the impact, to investigate its role in the treatment of ALI and possible mechanisms ALI / ARDS infection due to its clinical application to provide a theoretical reference. Methods: 56 SD rats were randomly divided into normal control group, LPS model for 2h, 4h, 6h, Tanreqing treatment 2h, 4h, 6h group, a total of seven groups, each group of eight. Normal control group were injected with sterile saline 1ml as a control, LPS model group I tail vein injection of LPS (5mg/kg) modeling, the treatment group Tanreqing to LPS model from another tail vein injection of phlegm-heat Qing 1mL treatment. Then in the corresponding period the rats were sacrificed after anesthesia to take blood, I lavage and lung tissue. Light microscope observation of lung tissue changes in the determination of lung wet / dry weight (W / D), calculated in bronchoalveolar lavage fluid (BALF) polymorphonuclear neutrophil ratio (PMN%), lung permeability was measured Index (LPI) to evaluate the degree of lung injury. Chemical detected in lung tissue homogenates of nitric oxide (NO), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) content changes. Immunohistochemical staining observed differential expression of the NF-κB p65 protein level. 5. SPSS17.0 statistical software to analyze process data. Results: Compared with normal control group, the lung tissue of LPS model group obvious morphological, pathological changes; lavage fluid PMN% lung W / D, LPI significantly increased (P lt; 0.01). Show that LPS can successfully copied the rat model of ALI. Compared with the corresponding period LPS model group, each time period lavage fluid of PMN%, lung W / D, LPI significantly lower (P lt; 0.01). 2. Than normal each time segment model lung tissue MDA and NO were significantly increased (P lt; 0.01), SOD and GSH-px were significantly decreased (P lt; 0.01). Tanreqing treatment of 4,6 h in lung tissue MDA and NO and the corresponding time period model group decreased significantly as compared (P lt; 0.01), lung tissue SOD, GSH-px activity was significantly higher (P lt; 0.01) . LPS model group of all time NF-κB p65 positive index values ??than normal group increased (P lt; 0.01). 2h group Tanreqing NF-κB p65 positive index with LPS model 2h group showed no significant difference (P gt; 0.05); Tanreqing 4h with a the 6h group of positive index value is significantly lower compared to the corresponding point in time model group (P lt ; 0.01), with no significant difference between the normal control group. Conclusion: 1. Tanreqing injection ALI rats have a protective effect, can reduce its morphological changes, reduce the degree of lung injury. 2. Tanreqing injection ALI rats protective effect may be related remove excess oxygen free radicals, improve the antioxidant capacity, and promote its tends dynamic equilibrium. Protective effect. Tanreqing injection ALI rats may be related to inhibition of the expression and activation of NF-κB, inhibit further transcription of various inflammatory cytokines.