The Protective Effects and Action Mechanism of Trans-resveratrol on Rats Hippocampus Injury by Aβ25-35
|School||Zunyi Medical College,|
|Keywords||Trans-resveratrol (TR) caspase-3 caspase-9 APP|
Objective To investigate the protective effects of trans-resveratrol (TR) on Aβ25-35 induced dementia rats and its mechanism.Methods After being trained by Morris water maze(MWM), rats were randomly divided into 6 groups:(1) sham-operated-group, (2) model-group, (3) TR low dose group, (4) TR middle dose group, (5) TR high dose group and (6)Aricept group. Dementia model was made by injecting Aβ25-35 to bilateral hippocampus of rats. Group (3)-(5) were treated with TR and aricept after the model was established. Aricept group was treated with aricept after the model was established. The level of spatial discrimination learning and memory of rats were tested with MWM. The level of caspase-3, caspase-9,APPmRNA expression in rats hippocampus and cortex were detected by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). The expression of caspase-9 protein levels in rats hippocampus and cortex were detected by Western blot.Results In the rats hippocampus after injection of Aβ25-35, the escape latency(s) of sham-operated-group, model-group, TR low-dose gorup, TR middle-dose group, TR high-dose-group, Aricept gorup 1 to 4 days MWM were (1)10.8±4.11,9.8±1.70,7.65±1.36, 6.65±1.82(s); (2) 81.11±10.27,74.81±6.43,68.48±7.7,64.31±4.7(s); (3) 45.80±9.5, 40.47±6.9,29.45±6.72,27.25±3.57(s); (4) 34.08±8.98,25.7±5.4,9.61±2.88,16.76±2.89(s); (5) 32.93±10.38,17.05±4.23,13.78±3.63,9.36±1.47(s); (6) 33.06±6.28,21.30±4.93, 14.20±2.72,18.44±3.12. Compared with other groups, the MWM escape latency of the model group was significantly prolonged(P<0.01). The caspase-3mRNA expressions detected in sham-operated-group, model-group, TR low-dose-group, TR high-dose-group, and Aricept group by qRT-PCR were 1.03±0.09,2.17±0.21,1.51±0.04,1.15±0.07, and 1.15±0.45; The caspase-9mRNA expressions detected in sham-operated-group, model-group, TR low-dose-group, TR high-dose-group, and Aricept group by qRT-PCR were 0.97±0.12,2.22±0.26,1.64±0.16,0.96±0.04,1.24±0.16; The APPmRNA expressions detected in sham-operated-group, model-group, TR low-dose-group, TR high-dose-group, and Aricept group by qRT-PCR were 0.94±0.14,2.0±0.15,1.5±0.17,1.08±0.06, and 1.19±0.05. Compared with those of other groups the caspase-3, caspase-9, APPmRNA expression of model group increased in rats’hippocampus (P<0.01). The caspase-9 protein expression detected in sham-operated-group, model-group, TR low-dose-group, and TR high-dose-group by Western blot were 0.66±0.99,1.37±0.18,1.10±0.10, and 0.98±0.50; Compared with those of other groups the caspase-9 protein expression of model group increased in rats hippocampus (P<0.01).Conclusion Treatment with different dose of TR can reduce Aβ25-35-induced memory impairment in rats. The mechanism may be partly associated with downregulating the mRNA expression of caspase-3,9, APP in rats’ hippocampus, control caspase-9 protein expression, and reducing the cell apoptosis in hippocampus.