Effect of Acupuncture and Minimally Invasive Hematoma Aspiration on Rabbit Brain Damnification in Acute Stage of Intracerebral Hemorrhage
|School||Dalian Medical University|
|Course||Traditional Chinese Medicine|
|Keywords||Acupuncture Minimally invasive hematoma aspiration Acute stage of intracerebral hemorrhage Matrix metalloproteinase-9|
Objective: Urgent phase of cerebral hemorrhage is a disease of high death rate. The ultimate measure of therapy are purge the haematoma in brain in time, control hydrocephalus and alleviate or reverse brain tissue damnification. Now the research of haematoma itself is not many, the therapy of internal medicine basically belongs to allopathy. The minimally invasive hematoma can prompt purge the haematoma, and is likely to the most prospect therapy method of urgent phase of cerebral hemorrhage. The curative effect of acupuncture had been legalized, but when intervention treatment is still be disputed. The text investigate the effect of acupuncture and minimally invasive hematoma aspiration to rabbit brain tissue damnification in urgent phase of cerebral hemorrhage to discuss whether has positive effect the acupuncture and minimally invasive hematoma for alleviate the hydrocephalus in urgent phase of cerebral hemorrhage or not. Accordingly new target and approach can be provided for the clinic of urgent phase of cerebral hemorrhage.Methods: A total of 65 healthy New Zealand white rabbits were randomly divided into a sham operation group (SO group, n=5),an intracerebral hemorrhage group (ICH group, n=20), a hematoma aspiration group (HA group, n=20), and an acupuncture and hematoma aspiration group (AHA group, n=20), the last three groups were further divided into groups of 6 hours, 24 hours, 3 days, 7 days(n= 5 in each group). The establishment of cerebral hemorrhage model reference to Zouwei and Deinsberger was produced after the success of 4h, once again anesthetized, the slow part of the intracranial injection of urokinase same 50μl (including UK2500u), 2 hours after the syringe gently with 1ml slow suction, the syringe was slowly pulled. The acupuncture and hematoma aspiration group :adopting nourish kidney, invigorate the circulation of blood and restore consciousness, we choose“Baihui”,“Shuigou”,“Fengfu”,“Taiyang”,“Xuehai”,“Taixi”,“Housanli”. Brain water content(BWC)was measured,and the expression of matrix metalloproteinase-9(MMP-9)in the rabbits’brains was detected by immunohistoehemistry.MMP-9 activity was detected by gelatin zymography.Results: The brain water content(WBC) of ICH group at every time were more than SO group, and peaked on the 3rd day(P <0.01).The BWC of HA group was obviously slower than ICH group at 6 hours and on the 3rd (P <0.01). The BWC of AHA group was significantly fewer than ICH group on the 6h、24h、3d and 7d(P<0.05 or 0.01).The BWC of AHA group descended significantly more than HA group as time went on(P<0.05),especially on the 3rd day in AHA group(P<0.01).Both Immunohistochemistry and Gelatin zymography showed that, the expression and activity of MMP-9 in the ICH increased at 6h after ICH and reached a peak at the 3rd day, afterwards, the expression of MMP-9 in ICH group reduced gradually. Compared with ICH group, the expression of MMP-9 in HA group and AHA group reduced at the 3rd day (P <0.01), and the expression of MMP-9 in AHA group reduced more than HA group(P <0.05).Conclusion: 1.The brain edema after urgent phase of cerebral hemorrhage is relevant to the expression of MMP-9;2.Acupuncture and hematoma aspiration may reduce cerebral hemorrhage in the expression of MMP-9, accordingly alleviate the intracerebral edema;3. Acupuncture can be used in urgent phase of cerebral hemorrhage.