Expression and Significance of Survivin、COX-2、VEGF in Non-small Lung Cancer Detected by Tissue Microarray Technology
|Course||Pathology and Pathophysiology|
|Keywords||Survivin COX-2 VEGF CD105 Carcinoma， non-small-cell Lung tissue microarray|
Objective: To study the expression and signifance of Survivin、COX-2、VEGF、CD105 in NSCLC and benign lesions tissue, and discuss the relationship among their expression. Method: The tissue microarray was made up of 71 cases of NSCLC tissue and 21 cases of benign lung lesions tissue. The expression of Survivin、COX-2、VEGF and CD105 were detected by immunohistochemistry. Results:①Survivin protein is expressed in 51 of 71(71.83%) cases of NSCLC, significantly higher than that of benign lesions(19.05%). There is a significant correlation between Survivin expression and TNM stages of patients (P<0.05);②There is a higher expression of COX-2 in NSCLC (77.47%) compared with benign lung lesions(14.29%). There is a significant difference between the expression of COX-2 and histologic subtype;③76.06% of NSCLC tissue expresses VEGF, the difference of the positive rate between NSCLC and benign lesions is significant. The expression of VEGF is related to tumor size and lymphnode metastasis;④The mean of MVD in NSCLC and benign lesions is (11.08+6.01) and (4.14+4.06) respectively, the positive correlation is found between MVD and lymphnode metastasis and TNM stages of patients;⑤There is positive correlation between Survivin and VEGF、MVD, Survivin and COX-2, COX-2 and VEGF、MVD. One of them increases with the other’s increasing in NSCLC. Conclusion:①The expression of Survivin、COX-2、VEGF and MVD is all significantly higher than that of benign lesions tissue, indicates that all of them facilitate the occurance and development in NSCLC;②The high expression of Survivin in NSCLC may serve as a reference prognosis marker; the significant high expression of COX-2 in pulmonary adenocarcinoma indicates that COX-2 perhaps promote hematogeneous metastasis in early stage; both VEGF and MVD can predict the NSCLC patients’ prognosis;③In NSCLC tissue,the positive correlation between Survivin and VEGF、MVD, indicates Survivin involve in tumor angiogenesis; the positive correlation between COX-2 and VEGF、MVD indicates COX-2 involve in tumor angiogenesis by enhancing the expression of VEGF; the positive correlation between Survivin and COX-2 suggests they all participate in the occurance and angiogenesis of NSCLC through coordination between them;④It is feasible to research large samples effectively and accurately by using tissue microarray.