Dissertation > Medicine, health > Basic Medical > Human morphology > Human histology

BMP2 and VEGF165 Genes Transfected Rat Bone Marrow Mesechymal Stem Cells Simultaneity Expression

Author ShiLei
Tutor TianXiaoBin
School Zunyi Medical College,
Course Surgery
Keywords bone morphogenetic protein 2 vascular endothelial growth factor rat bone marrow mesenchymal stem cells
CLC R329
Type Master's thesis
Year 2011
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Objective:To establish the method of isolation and cultivation of rat bone marrow mesenchymal stem cells(BMSCs) in vitro.To evaluate the simultaneity expression efficacy of rat bone marrow stromal cells(BMSCs) transfected with bone morphogenetic protein 2(BMP2) and vascular endothelial growth factor(VEGF) genes.Methods:BMSCs from rats were isolated and cultured in vitro by using the whole bone marrow adherence method.The BMSCs were transfected with BMP2 and VEGF165 by liposome-mediated method.The co-espression of BMP2 and VEGF165 genes were determined by Enzyme-linked immuno sorbent assay (Elise) and immunohistochemical analysis.Results:The cells transfected with BMP2 and VEGF165 indeed could produce the proteins of BMP2 and VEGF165.Conclusion:the BMSCs transfected with BMP2 and VEGF165 genes will be expression simultaneously of rat bone marrow mesenchymal stem cells.

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