Dissertation
Dissertation > Environmental science, safety science > The basic theory for the Environment and Science > Environmental Chemistry > Environmental Analytical Chemistry

Research on Adsorption and Degradation of Triphenyltin in Water Environment by Microorganism

Author ShiYiZhi
Tutor YinHua
School Jinan University
Course Environmental Engineering
Keywords organotin trephenyltin Klebsiella pneumoniae biosorption biodegradation enzymatic degradation
CLC X132
Type Master's thesis
Year 2009
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The biosorption and biodegradation of triphenyltin(TPhT) was studied in the research, including selection of biosorption and biodegradation strain,capability of sorption and degradation,and mechnisam of biodegradation.The high-performance liquid chromatography ultraviolet detector method for detection of triphenyltin in sediment was established,the optimal wavelength was 206nm,and flow phase was mixed liquid of methanol,pure water and trifluoroacetic acid of one percent at the volume ratio as 50:45:5.Ethyl acetate was used to extract TPhT from water sample,with the recovery of 78.8%~126.5%when the concentration of TPhT was 0.5~3.0mg/L.The sediment of 2g,which was collected from Lianjiang River,Guiyu Town,Guangdong Province,was added by 3mL 6mol/L HCl and 25mL ethyl acetate,shaked for 30min in the constant temperature shaker at the speed of 140 r/min,then ultrasonic extracted for 15min.Finally the concentration of TPhT in this sediment was 0.94±0.33mg/kg.The bacterial strain N-5 possessed the best properties of toxicity resistance to triphenyltin and exhibited much better biosorption and degradation ability of TPhT,and also could grow well using TPhT as sole carbon source.The strain was identified as Klebsiella pneumoniae through physiological and biochemical experiments and 16SrDNA sequence analysis.The degradation rate was 14.1%~65.4%within 5d when bacteria concentration was 2 to 20g/L.The optimal degradation conditions were as follows:the culture time of microorganism was 36h,the concentrations of bacteria,TPhT and additional glucose was 14g/L,5mg/L,5mg/L,respectively.Bacterial cells of N-5 changed a lot and tended to aggregate together in TPhT polluted environment.The process of biodegradation on TPhT occurred intracellularly,including biosorption by cell wall,active transportation across cell mambrane,and biodegradation within cytoplasm.Also 28.1%to 77.8%of TPhT would be degraded by separated endoenzyme using 2 to 20g/L biomass of K.pneumoniae within 2 h,with the optimal conditions including degradation time 2h,TPhT concentration 5mg/L,temperature 50℃,pH 8,and concentration of Mn2+,Mg2+, Fe2+,Fe3+ being 10,15,10,5mg/L,respectively.Preliminary identification showed that albumen of endoenzyme fluid of bacteria was composed ofα-amino and tyrosine residue with hydroxylation of phenol etc.,ultraviolet-visible maximal absorptive wavelength was 300~500nm.

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