Evaluation the Improvement of Cardiac Function with Myocardial Infarction Transplanted into Autologous Bone Mesenchymal Stem Cells from Different Ways by Ultrasound
|School||Dalian Medical University|
|Keywords||marrow stromal cells transplantation acute myocardiac infarcti|
Objective: In order to solve the problem of the conventional treatments of acute myocardial infarction (thrombolytic therapy, interventional therapy, heart bypass surgery) that can not make the myocardial regeneration, can not limit ventricular remodeling and can not reduce the heart failure risk caused by myocardial infarction, it is urgent to find a new method—stem cell transplantation. Recentely, there were several ways in which the stem cells being transplanted into the rabbits with myocardial Infarction, such as, intravenous way, percutaneous interventional treatment, and myocardium way. Whether or not the efficacy of those ways having differences there was no conclusion. Therefore, we carry out this experiment to evaluate the improvement of cardiac function with myocardial infarction transplanted into autologous bone mesenchymal stem cells on rabbits from intravenous way or myocardium way.Methods: Myocardial infarct models were set up by ligating the left anterior descending coronary artery. 60 New Zealand rabbits myocardial infarct models were randomly divided into two experimental groups (20 rabbits/per group) and two control groups (10 rabbits/per group). One week after the ligation, the experimental groups were injected with autologous MSCs through direct injection into myocardium and intravenous injection. the control groups were separately treated with an equal volume of cell-culture medium through direct injection of vein and myocardium. To evaluate the cardiac function, echocardiography was performed in turn from pre-ligation, after the ligation, the first week after the transp1antation to the forth week after the transp1antation, to measure the LVΔT and LVFWSD, and to calculate left ventricular eject fraction. M type echocardiography indicated that the fourth week after transplantation, the left ventricular function was increased significantly both in intravenous and myocardium trailsplanting group (P<0.05). There was no diffrence between the intravenous group and myocardium transplanting group(P>0.05).Result:(1)Echocardiography indicated that after the ligation , the values of LVEF, LVFWSD and LVΔT all obviously reduced both in experimental groups and control groups (P<0.05).(2)There were no improvement of LVEF, LVFWSD and LVΔT values on the first week after transplantation than after the ligation both in intravenous and myocardium trailsplanting group(P>0.05).(3)The values of LVEF, LVFWSD and LVΔT were great improvement the forth week after transplantation than after the ligation both in intravenous and myocardium trailsplanting group(P<0.05).(4)There were no statistic diffrence between intravenous and myocardium trailsplanting group on the values improvement of LVEF, LVFWSD and LVΔT both on the first and forth week after the ligation(P>0.05).(5)Echocardiography indicated that the forth week after transplantation the values of LVEF, LVFWSD and LVΔT all obviously improved than after the ligation , both in intravenous and myocardium trailsplanting group(P<0.05).(6)Cardiac function was worse on the forth week after transplantation than pre-ligation both in intravenous and myocardium trailsplanting group (P<0.05).Conclusion:Both of the two ways by which the autologus bone marrow cel1s were transplanted into the the rabbits with myocardial infarction can relieve left ventricu1ar remodeling and improve 1eft ventricular cardiac function after 4 weeks from transplantation. There was no diffrence between the intravenous group and myocardium transplanting group in improving the left ventricular function.