Study on the Correlation Between the Levels of Il-18, IL-33 and Other Inflammatory Factors and Pulmonary Function in Different Stages of Asthma
|School||Dalian Medical University|
|Keywords||interleukin(IL)-18 interleukin(IL)-33 bronchial asthma pulmonary function test|
Objective: To investigate the levels of some inflammatory factors including interleukin（IL）-18, interleukin（IL）-33, eosinophils（EOS）, immunoglobulin（Ig）E, interferon（IFN）-γ, high sensitive C-reactive protein（hs-CRP） and pulmonary function in bronchial asthma patients. Study on the correlation between the level of those factors and the level of pulmonary function,and analyze the role of IL-18 and IL-33 in clinical diagnosis.Methods: The blood samples and clinical data from the 45 asthma patients in acute attack period and in remission period were collected. The serum levels of IL-18, IL-33, IgE, IFN-γwere detected by the method of ELISA. Also the data of the blood routine test and high sensitivity C-reactive protein were collected. While the blood samples of 25 healthy non-asthmas people were also collected to study as a control group. All patients were taken pulmonary function tests before and after glucocorticoid treatment to determine the level of FEV1, FEV1%pre, FEV1/FVC%, FEF50%pre, FEF75%pre, FEF25-75%pre and Raw%pre. Correlation between the inflammatory factors such as IL-18 and IL-33 and lung function were analyzed as well.Results:The results in our study were as below: first, the level of serum IL-18 in asthma patients was significantly higher than that in control group（P<0.05）, and the level of serum IL-18 in acute asthmatic attack period was significantly higher than that in remission period （P<0.05）; second, the levels of serum IL-33 and IgE in acute asthmatic attack period were significantly higher than those in control group（P<0.05）, and the level of serum IL-33 in acute asthmatic attack period was significantly higher than that in remission period （P<0.05）; third, the levels of serum hs-CRP in patients with acute asthmatic attack period was significantly higher than that in control group（P<0.05）; fourth, the levels of serum IL-18, IL-33, IgE, EOS,EOS%, hs-CRP in asthmatic patients were positive correlation; fifth, the pulmonary function indicated that the levels of FEV1, FEV1%pre, FEV1/FVC%, FEF50%pre, FEF75%pre and FEF25-75%pre in acute asthmatic attack period was significantly higher than that in remission period （P<0.05）; and the levels of FEV1 and other pulmonary function index in patients after glucocorticoid treatment were significantly higher than pretreatment（P<0.05）, whereas the level of Raw%pre decreased significantly than pretreatment（P<0.05）; sixth, the prominent negative correlations were seen between the levels of IL-18, IL-33 and the level of FEV1%pre both in acute asthmatic attack period and in remission period; meanwhile, there were significant difference of the levels of IL-18 and IL-33 in mild group, moderate group and severe group of pulmonary function; and there were distinct negative correlations between the levels of IgE, hs-CRP, eosinophils and the level of FEV1%pre.Conclusions: IL-18 and IL-33 are related to the pathogenesis of asthma. And distinct correlations exist between the levels of IL-18, IL-33 and pulmonary function in asthma patients. Thus, these inflammatory factors could be used to effectively evaluate the severity of asthma.