Vitis Vinifera L. Cell Cultures and the Secondary Metabolic Regulation of Resveratrol
|School||Gansu Agricultural University|
|Course||Crop eco- physiological|
|Keywords||Vitis vinifera L. cell culture resveratrol metabolic regulation|
Resveratrol, a known antioxidant compound naturally produced in plants as a self-defense agent in response to exogenous stimuli such as pathogen attack, ozone treatment, pre-harvest and post-harvest UV-C irradiation, etc, belongs to the phenolic phytoalexins group and stilbenes. Resveratrol has many biological activities, i.e. estrogenic activity, cardiovascular protective effect, neuroprotective capacity, and cancer chemopreventive activity. The treatment of plant cells with biotic and abiotic elicitors has been one of the most effective means to improve the yields of secondary metabolites in plant cell cultures. In our study, weighing method for growth, organic solvent method for extraction and HPLC method for determination were used to study the influences of supplementary materials （phenylalanine, cinnamic acid, salicylic acid,β-cyclodextrin, colchicines and silver nitrate）, carbon sources （sucrose, glucose and maltose）, light quality （red, yellow, blue, green and white light）, UV-C treatment and harvest time on the growth of the callus from Pinot Noir and its resveratrol accumulation. The results showed that 30, 30, 40, 40, 5, 30 mg·L-1 are the optimal concentrations in turn for resveratrol-forming; except colchicines, the other additions did not show any stimulative effect on the multiplication, and cinnamic acid, salicylic acid and silver nitrate inhabited its growth. Sucrose was superior to the other two for the multiplication but inferior to those for resveratrol accumulation, however, as the osmotic pressure increased, only sucrose can enhance the callus growth and resveratrol-forming which was suppressed by glucose and maltose. The order of multiplication effects from the high to low was yellow, green, red, blue, white light, and that of resveratrol accumulation was white, yellow, red, blue, green, but resveratrol was not detected in the callus growing under green light. With the treatment of UV-C, longer periods of irradiation considerably weakened the capacity of the calli to synthesis resveratrol, and with exposure time of 10min, maximal induction took place (104μg·g-1); as the intensity of the light increased, the content of resveratrol was enhanced obviously, 0.4 W·m-2 made the content of resveratrol increase to 170μg·g-1, 4.2 and 3.3 times more than the control and 0.05 W·m-2 did respectively. 9 days after the inoculation of the callus, 64.5μg·g-1 resveratrol formed, which was the highest content in the whole growth period, and only 3.7μg·g-1 was detected on the 45th day.