Endoplasmic Reticulum Stress and the Expression of Cyclin D1, HGF Receptor C-met in Mouse with Acute Liver Failure
|School||Zunyi Medical College,|
|Keywords||liver failure acute Endoplasmic reticulum stress Cyclin D1 HGF receptor|
Backgrounds and Aims:Patients with liver failure had impaired liver regeneration and high mortality without liver transplantation. However, the mechanisms involved in the impaired liver regeneration remained unclear. The aims of this study were to explore the possible correlations of endoplasmic reticulum stress (ER stress) with impaired liver regeneration in mice with acute liver failure (ALF).Methods:Mice with ALF were developed by intraperitoneal injection of D-galactosamine (D-Gal) and lipopolysaccharide (LPS). Biochemical parameters in serum and pathological changes in liver tissue were detected at different times. BIP/GRP78, Cyclin D1 proteins of livers were determined by Western bolt. The expression of HGF receptor c-met mRNA in liver was detected by reverse transcriptase PCR (RT-PCR).Results:3 hours after intraperitoneal injection of D-Gal and LPS, the level of serum ALT and AST began to increase and reached the peak 7 hours later, while massive hepatocyte necrosis were observed. The indicators of endoplasmic reticulum (ER) stress, BIP/GRP78 proteins expression in the livers increased significantly from 3 to 7 hours after administration of LPS and D-Gal, however, the expression of Cyclin Dl proteins and HGF receptor c-met mRNA deceased markedly.Conclusion:our results demonstrated that ER stress persistently existed in hepatocyte and correlated to decreased expression of CyclinDl proteins and the mRNA of HGF receptor in mouse with ALF, indicting that ER stress of hepatocyte maybe one of factors related to impaired liver regeneration in mouse with ALF.