Expression Characteristics and Clinical Significance of RECK in Cervical Cancer
|School||Dalian Medical University|
|Course||Human Anatomy and Embryology|
|Keywords||Cervical cancer RECK Protein expression Immunohistochemistry|
Backgrounds and Objectives: The changes and remodeling of extracellular matrix (ECM) is the essential factors in tumor invasion, metastasis and remodeling. Invasion and metastasis have to break through the barrier of ECM and basilar membrane. Classics molecule matrix metalloproteinases are endogenous peptidase containing Zn2+ matrix, which degradating the ECM including collagen protein. Elastin, glycoprotein and glycoprotein, and belongs to hydrolase. RECK gene was found in 1998 with the inhibition function of MMP expression and activation. Thus to inhibit the invasion and metastasis od malignancies. RECK gene regulates the MMP-2, MMP-9 and VEGF to protect the ECM from degration to prevent tumor progression.Cervical cancer (CC) is the most common malignancy of female in developing countries, with the highest morbidity among all kinds of female genital organ tumors. 500 thousand of new cases per year occur and 5 year survival rate is always around 52%. 270 thousand women die of this disease, 85% of whom are from developing countries, with youth tendency. So CC is a large threatening for female health and life. The multi-gene, multi-steps changes induce the carcinogenesis of CC, from untypical hyperplasia, cancer in situ, early invasive cancer and invasive carcinoma as a continuous process. The conjunction of squamous and columnar epithelia is the location for CC. Current study proved virus including human papilloma virus (HPV) is the key causes for the cancer because 99% of patients were effected. Meanwhile the abnormal activation of oncogene and inactivation of anti-oncogene relate to the tumor formation of CC, but there are less research on the relation of RECK expression and CC progression.This research aims to detect the expression characteristics of RECK in CC and profile the relationship to the series of pathological factors through the methods of tissue array and immunohistochemistry staining. Materials and Methods:CC and relative normal tissues were all selected from China Medical University, Oncology Hospital of Liaoning province and Army 463 Hospital. 56 CC samples were taken after operation and 33 cases of relative normal cervix were from hysteromyoma patients. By the methods of paraffin embedded tissue array immuno- histochemistry, the expression pattern of RECK in different cervical tissues were detected, the correlation between RECK expression and CC pathology was analyzed. The data were statistically analyzed by Kruskal-Wallis, Mamm Whitney and Spearmen with SPSS 11.0 software.Results:1. 97% of normal cervical tissues express RECK protein however 78.6%(37/56) of CC showed down-regulation of RECK expression, and statistical analyses with Kruskal-Wallis test and Mann-Whitney test revealed the significant differences between the two groups(P=0.000). 2. No statistical differences could be observed in different, age group and different pathological types. Frequent loss of RECK expression low differentiation level compared with the high (p=0.001).Conclusion: 1. The expression of RECK gene is an important molecular event throughout the CC carcinogenesis. The expression frequency decreases from noncancerous to tumor.2. The frequency of RECK loss of low differentiation level is significant compared with that of the high, but unrelated to factors of, age and pathological types which show potential oncology significance.