Scopolamine on Different Phases of Object Recognition Memory in Mice
|School||Capital Normal University|
|Keywords||Object recognition Scopolamine Naltrexone Working memory|
Object recognition task (object recognition test, ORT) to measure the behavior of the animal non-spatial working memory model is in its natural state, the model including adaptation period, the familiar period and test period. Adapt to the box and hold phase behavior of object recognition, the animals adapt to three days, and then into the familiar period, when two identical object recognition, recording animal behavior box placed to explore two objects of the time delay period of time after the test period, which when for an old object to the new object, record the animal time to explore the old and new objects. Experiment 1 The purpose of this study was to establish a mouse object recognition model to explore the characteristics of object recognition memory in mice weakened over time. The results show that: 1h and 3h the intervals group of mice of the old and new objects distinguish index exist significant differences (P <0.05) after the end of the training, the animals maintain good 1h and 3h memory, object recognition: the delay time of 4h and 24h animal familiar phase identification Old object can not remember, no significant differences in the test phase of the old and new objects to explore time (to distinguish index close to zero). Distinguish memory in mice of the old and new objects familiar after forgetting delay between 3-4 hours. Observed in experiment 2 scopolamine on object recognition memory access, maintain, extract the three aspects of the impact and role of object recognition memory impairment induced by scopolamine, naltrexone. The results showed that, given scopolamine (1mg/kg, ip) 20 minutes ago familiar damage the encoding of object recognition memory in mice, object recognition memory acquisition stage (P <0.05), but familiar with the period immediately after administration and 20 minutes before the test administration did not harm the object recognition memory in mice that have no effect on the memory retention and the extraction stage. Finally, in the three stages of the object recognition memory, while giving scopolamine and naltrexone (1mg/kg, ip), results show that the link in the information encoded 1mg/kg naltrexone compared to a single injection of scopolamine increased in mice the resolving power of the old and new objects, but not with a single injection of scopolamine group significant differences; and object recognition memory retention and extraction with a single injection of scopolamine group there was no significant difference. The role of naltrexone improve memory needs to be done further research.