Dissertation
Dissertation > Medicine, health > Oncology > Gastrointestinal Cancer > Gastric neoplasms

Research of Expression and Clinical Significance of p53、PCNA and PTEN in Gastric Cancer

Author JiaHaiJiang
Tutor DaiLin
School Dalian Medical University
Course Internal Medicine
Keywords gastric cancer p53 PCNA PTEN clinical significance
CLC R735.2
Type Master's thesis
Year 2011
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Objective:Gastric cancer is one of the most common malignant tumors. Development of gastric cancer related to many factors including activation of oncogene, inactivation of tumor suppressor genes and tumor metastasis suppressor gene. Meanwhile, abnormal expression of these oncogenes affected the prognosis of gastric cancer patients. In this study, immunohistochemical SP methods were used to detect the expression of p53, PCNA (Proliferating Cell Nuclear Antigen) and PTEN (phosphatase and tensin homolog deleted on chromosome ten) protein in resected gastric cancer tissues. The relationships between these genes and the biological characteristics of gastric cancer or the prognosis of gastric cancer patients was analyzed and their value was evaluated to determine the prognostic of gastric cancer patients.Methods:Mouse anti-human p53, PCNA, PTEN monoclonal antibody were used to in immunohistochemical staining of of surgically resected gastric cancer specimens from 55 cases to observed expression in gastric cancer cases of p53, PCNA and PTEN gene. By SPSS 13.0 statistical software, theχ2 test was used to analyze the relationships of p53, PCNA, PTEN expression and biological characteristics and prognosis of gastric cancer.Results:1. Positive expression of p53, PCNA and PTEN are all localized in the nucleus in gastric cancer cells. Positive rate of p53 expressio was 58.2%; Strong positive rate of PCNA expression was 52.7%; Negative rate of PTEN expression was 52.7%.2. p53, PCNA, and PTEN expression was related wiht the depth of tumor invasion and lymph node metastasis (all P<0.05) and was not related with the degree of tumor differentiation (all P>0.05). 3. Gastric cancer patients with p53 expression had less probability of 5-year survival than patients with negative expression; Gastric cancer patients with strong PCNA expression had less probability of 5-year survival than patients with weakly expression; Gastric cancer patients with PTEN expression had more probability of 5 years survival than patients with negative expression.The differences were all statistically significant (P<0.05).4. Gastric cancer patients with 0-1 abnormal expression of the above genes had less probability of invasion to serosa-serosa outside than patients with 2-3 abnormal gene expression. Gastric cancer patients with 0-1 abnormal gene expression had less probability of lymph node metastasis than patients with 2-3 abnormal gene expression. The differences were both statistically significant (P<0.05).5. Gastric cancer patients with 0-1 abnormal genes expression had more probability of 5-year survival than patients with 2-3 abnormal gene expression. (P <0.05).Conclusion:1. Expression of p53, PCNA or PTEN were all related to the depth of tumor invasionand metastasis of lymph node.2. Gastric cancer patients with positive expression of p53, strong positive of expression PCNA or negative expression of PTEN had poor prognosis.3. Gastric cancer patients with more numbers of abnormal gene expression had more probability of tumor deep invasion, lymph node metastasis and poor prognosis.4. The combined detections of p53, PCNA, and PTEN expression in gastric cancer specimens might be used as one of the indicators to determine the prognosis of gastric cancer patients.

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