Relationship between Expression of COX-2 and Cell Cycle Regulatory Proteins in Patients with Esophageal Squamous Cell Carcinoma
|Keywords||Esophageal cancer Cyclooxygenase-2 Ki-67 p27 protein Cyclin A|
Objective To investigate the cyclooxygenase -2 (COX-2) and cell cycle regulatory proteins (Ki-67, cyclin A, p27) in esophageal squamous cell carcinoma (ESCC) Expression and correlation. ESCC randomly selected after radical resection specimens 102 cases, 28 cases of adjacent normal squamous epithelium as controls. Immunohistochemical detection of each case ESCC and adjacent normal tissue COX-2, Ki-67, cyclin A (cyclin A) and p27 expression, using a unified assessment method: positive tumor cells staining score = percentage (0 -100 points) × tumor staining degree (0 negative, weakly positive in 1 minute, 2 minutes moderate, strong positive 3 points), score value range is 0-300 minutes, the results from the average of five view as the final result; analysis of variance, analysis of them with ESCC clinical pathological factors; pearson correlation analysis and the use of their relevance in ESCC, P lt; 0.05 was considered statistically significant. Results (1) COX-2 in normal esophageal squamous epithelium and ESCC mean staining scores were 30.2,74.7, the expression in ESCC, the difference was statistically significant (P lt; 0.01). (2) Ki-67 in normal esophageal squamous epithelium mean staining score was 11.6, in ESCC was 64.0, higher than the normal esophageal squamous epithelium (P lt; 0.01), that is, in ESCC has high Ki-67 expression. (3) in cyclin A expression in ESCC mean staining score of 44.2, higher than the normal esophageal squamous epithelium 11.7 (P lt; 0.01), that is, increased expression of cyclin A in ESCC. (4) p27 mean staining scores in the normal esophageal squamous epithelium and ESCC were 266.4 and 87.7, that p27 expression in ESCC was significantly reduced (P lt; 0.01). (5) COX-2 expression and tumor T-staging, T1-2 T3-4 Expressing below 期 (55.0 ± 42.3vs83.0 ± 66.5, t = -2.62, P lt; 0.05), COX-2 with other clinicopathological factors. (6) Ki-67, cyclin A, p27 and cell differentiation (F = 7.839, P lt; 0.01, F = 3.519, P lt; 0.05, F = 5.49, P lt; 0.01), Ki-67, cyclin A , p27 and other clinicopathological factors. (7) Person correlation analysis showed that, COX-2 and Ki-67, cyclin A expression was positively correlated (r = 0.270, P lt; 0.01, r = 0.233, P lt; 0.01), and p27 (r = -0.311, P lt; 0.01) negatively correlated with expression. Conclusions (1) COX-2, Ki-67, cyclin A expression in ESCC than in adjacent normal squamous epithelium, p27 expression in ESCC than in adjacent normal squamous epithelium. (2) COX-2 expression and tumor T-staging, COX-2 expression in ESCC may reflect the degree of tumor cell invasion. (3) Ki-67, cyclin A, p27 expression and cell differentiation, may reflect the degree of tumor cell proliferation. (4) COX-2 and Ki-67, cyclin A high expression and low expression of p27 correlated, suggesting that COX-2 overexpression ESCC cell proliferation are closely related.