Study on the Correlation btween Serum, Aqueous Humor VEGF, IL-6 Levels and the Neovascular Glaucoma
|School||Dalian Medical University|
|Keywords||Neovascular glaucoma Vascular endothelial growth factor Interleukin-6|
Objective: neovascular glaucoma (neovascular glaucoma, NVG) is a complex etiology, clinical manifestations severe refractory, intractable glaucoma, is a common cause of blindness. Usually secondary to posterior segment extensive ischemic diseases such as diabetic retinopathy (diabetic retinopathy, DR), retinal vein occlusion (retinal vein occlusion, RVO) and so on. The main clinical features NVG difficult to control high intraocular pressure and severe eye pain, but with conventional glaucoma treatment is often ineffective, and ultimately often leads to severe loss of visual function even enucleation and other consequences. However, early diagnosis, aggressive prevention and effective treatment and other measures to control the disease can develop and retain good visual function purposes. Currently, for NVG specific pathogenesis is not clear, but studies at home and abroad, but it never stopped. As the molecular biology and immunology of development, with a number of angiogenesis-related cytokines and their receptors more and more attention to the academic ophthalmology. Vascular endothelial growth factor (vascular endothelial growth factor, VEGF) is a kind of specifically stimulate endothelial cell proliferation and angiogenesis platelet-derived proteins. Interleukin -6 (interleukin, IL-6) is the acute phase of infection or trauma-induced stress responses major factor, but also a regulation of inflammatory responses multifunctional cytokine. The purpose of this experiment by testing serum and aqueous humor NVG VEGF and IL-6 levels were observed between the two and their relationship with the NVG, to further clarify the angiogenic growth factors on the incidence of NVG, revealing the possible onset of NVG mechanism; and through angiogenic factors in serum and aqueous humor Correlation, trying to find the intraocular fluid can reflect the level of indicators related factors for early clinical prediction, evaluation and prognosis of the disease provide a theoretical basis. Methods: 20 patients with NVG as the experimental group (A), primary chronic angle-closure glaucoma patients (B group) as a control group, 20 cases of ocular hypertension, senile cataract patients (C group) as a normal control group of 20 case. Three groups of patients collected serum and aqueous humor samples by double antibody sandwich ELISA method (enzyme-linked immuno sorbent assay, ELISA method) were detected in serum and aqueous humor VEGF, IL-6 concentration levels. Compare VEGF, IL-6 levels in the differences between the groups; analysis of each group of VEGF and IL-6 in correlation; study groups VEGF, IL-6 in serum and aqueous humor concentration difference between their correlation. Application SPSS17.0 statistical software processing of data, the same factor within the same group of data between different specimens compared with independent samples t-test; All data have homogeneity of variance, the factors between different groups compared using analysis of variance (ANOVA). Correlation analysis using Pearson correlation coefficient test. P lt; 0.05 indicates a statistically significant difference. Results: 1 in each group VEGF, IL-6 concentration levels: ① levels of VEGF in the aqueous humor: A group (1336.80 ± 70.15) pg / ml was significantly higher than that in group B (311.60 ± 31.06) pg / ml and group C ( 165.75 ± 13.95) pg / ml, pairwise comparisons among the three groups, the differences were statistically significant (F = 4019.334, P lt; 0.05). ② aqueous humor levels of IL-6: A group (691.15 ± 50.09) pg / ml was significantly higher than that in group B (168.25 ± 11.95) pg / ml and C group (92.10 ± 9.59) pg / ml, three between any two groups comparison, the differences were statistically significant (F = 2275.019, P lt; 0.05). ③ serum levels of VEGF: A group (545.40 ± 155.49) pg / ml than in group B (321.15 ± 52.57) pg / ml and C group (176.30 ± 20.38) pg / ml, pairwise comparisons among the three groups, the difference were statistically significant (F = 75.940, P lt; 0.05). ④ serum levels of IL-6: A group (291.35 ± 22.66) pg / ml than in group B (104.35 ± 13.21) pg / ml and C group (87.00 ± 12.70) pg / ml, pairwise comparisons among the three groups The differences were statistically significant (F = 906.947, P lt; 0.05). 2.NVG group of aqueous humor VEGF and IL-6 levels showed a significant positive correlation, the difference was statistically significant (r = 0.857, P lt; 0.05). The remaining specimens in each group had no significant correlation (P gt; 0.05). 3.NVG group of patients in the aqueous humor VEGF, IL-6 were significantly higher than in serum, and the difference was statistically significant (t = -20.748, P lt; 0.05; t = -32.199, P lt; 0.05). 4 in each group of serum VEGF, IL-6 concentrations and the corresponding aqueous humor showed no significant correlation (P gt; 0.05). Conclusions: 1. VEGF, IL-6 may promote the occurrence and development of NVG. 2. VEGF, IL-6 during the formation of the NVG, having mutually synergistic effect. 3. NVG may exist ocular VEGF, IL-6 production and a separate mechanism.