Dissertation
Dissertation > Medicine, health > Oral Sciences > Oral and maxillofacial surgery > Oral and maxillofacial plastic surgery

Study on the in Vitro Effect of Zinc on the Growth of Murine Embryonic Palatal

Author MouLuRui
Tutor HuangGuiLin
School Zunyi Medical College,
Course Clinical Stomatology
Keywords Mice Zinc All-trans retinoic acid Vitro Embryonic palate sudden
CLC R782.2
Type Master's thesis
Year 2011
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Objective: To understand the different concentrations of zinc on cultured mouse embryonic palate sudden impact fertility , explore suitable murine embryonic palate burst sterile best zinc concentration ; build all-trans retinoic acid -induced cleft palate in vitro model to explore the zinc in the whole trans retinoic acid -induced fetal cleft palate in the process of antagonism. Method : Get 13 days C57BL/6N embryonic fetal palatal cultured in vitro , according to different factors will interfere with embryo cultured palate suddenly divided into control group (A : ZNO} μmol / L), low zinc group (B group : ZN50μmol / L), zinc (C group : ZN75μmol / L), high zinc group (D group : ZN95μmol / L), comparative analysis of different concentrations of zinc on cultured embryonic palate fusion sudden impact . According to the previous palate embryos obtained promote optimal zinc concentration process fusion understand ATRA group (E ATRA10μmol / L) and all-trans retinoic acid (ATRA10μmol / L) optimal zinc concentration (F group ) on the palate embryo process fusion effect for 24 hours, 48 ??hours after the removal palate embryo sudden , asanas morphological changes were observed under a microscope and taking into account recorded a sudden embryonic palate fusion , cultured for 48 hours after HE staining understand the fusion process . Results : A, B, C, D, E, F group palate embryo process fusion rates were 27.8% , 53.9% , 85% , 32%, 0% and 6.7% respectively. A, B, C, D each pairwise comparison between groups was found parallel χ2 test , A group , B group and D group process fusion between the embryonic palate was no significant difference (P gt; 0.05), while Group C with significant differences between the groups (P lt; 0.05), ZN75μmol / L significantly promote the role of palatal fusion is the process fusion in vitro embryonic palate in the optimal concentration . The incidence of cleft palate in group F , although lower than the E group , but the group E and F group was no statistically significant difference (P gt; 0.05). Conclusions: ( 1 ) Palate vitro can be basically repeated contact with the body palatal fusion process . ( 2 ) embryonic palatal zinc concentration on the development of sudden impact , 75μmol / L concentration of zinc can be effective in promoting embryonic palatal fusion protrusion , which is the optimal concentration of zinc in vitro . ( 3 ) successfully established all-trans retinoic acid -induced cleft palate in mice in vitro model , 75μmol / L of zinc on 10μmol / L all-trans retinoic acid -induced cleft palate without antagonism.

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