Dissertation
Dissertation > Agricultural Sciences > Plant Protection > Pest and Disease Control > Crop pests and diseases and their prevention > Economic crop pests and diseases > Medicinal crop pests and diseases

Pathogen Identification and Pesticides Screening Indoor for Diseases of Saposhnikovia Divaricata (Turcz.) Schischk

Author XueWei
Tutor GaoJie
School Jilin Agricultural University
Course Plant Pathology
Keywords Wind New Disease Identification of the pathogen Biological characteristics Fungicide screening
CLC S435.67
Type Master's thesis
Year 2008
Downloads 149
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Reported wind bacterial soft rot, bacterial leaf rot, stem blight in the world occurred for the first time, wind leaf spot, powdery mildew, leaf blight occurred in Jilin Province, the detailed description of the six kinds of disease symptoms and hazardous conditions and to which the biological characteristics of the two fungal diseases, the preliminary research and the prevention and treatment of all diseases of Pharmacy indoor screening. Wind bacterial soft rot main hazards stem base and underground roots, the formation of brown, water-soaked rot, after gradually extended to the entire root cause soft rot and foul-smelling mucus flow issued diseased slightly pull that since. Bacteria the bacterial rod-shaped, Gram-negative (G -) weeks flagella to multi-root, facultative anaerobic, capable of growing at 37 ℃, 5% NaCl growth, not reducing substances from sucrose , malonate, can not be used to make potato soft rot, gelatin is not liquefied, DNA GC mol% and 53.2%, with reference to the relevant literature the identified as carrot pectin bacilli of carrot Asian Pectobacterium carotovorasubsp.carotovora (Jones 1901) Bergey et al.1923. The wind and bacterial leaf rot major damage leaves, the main symptoms of leaf necrosis, shrinkage and deformity. Bacterial RODS, Gram stain negative reaction (G -), a polar flagella, can not grow at 41 ℃, oxidase negative, potato soft rot, arginine dihydrolase negative reaction, fruit polyethylene sugar to produce negative, positive gelatin hydrolysis, nitrate reduction negative, and can use glucose, tartaric acid, and sorbitol as the sole carbon source, can not use sucrose, trehalose, β-alanine, and geraniol, as a carbon source, can take advantage D (-) tartrate, DNA of GC mol% to 58.3%. The control \Windproof Septoria major hazard leaves, early onset of leaf surface raw brown small point, after the expanded diameter of 2 to 5mm brown, the central part is paler in color, dark brown edges, circular or near-circular lesion with Physalospora , raw black arabica point and that pathogen conidia. Needle-shaped conidia, base obtuse, colorless, transparent, upright or slightly curved, the top slightly sharp inner oily ball size (18 -) 20 ~ 45 (-48) × 1.5 (-1.7) ~ 2.5 ( -3.1) μm. According to the morphological characteristics of the wind pathogenic pathogens, causing symptoms characteristic of the disease, with reference to the relevant literature was identified for the wind Septoria Septoria saposhnikoviae GZLu et JKBai. Windproof the powdery mildew major hazards blades of the wind. The blade on both sides of the formation of a white powdery spots, grow dark brown small point, late in the powder spot that pathogens closed capsule shell. Leaves gradually turn yellow dry. The conidia nearly cylindrical size (20.4-) 25.3 to 36 (-40.6) × (10.0-) 12.5 - 16.2 (-17.8) μm. Closed capsule shell scattered to nearly Poly Health, dark brown, flat spherical sub wire (-8) to 38 (-48) root, nearly two-forked or irregular branches. Months, nearly oval, the ascospore (2 -) of the ascus 2-4 (-6) 3 ~ (6 -) 8, oval to elliptical. Caused the morphological characteristics of the disease symptoms and pathogen characteristics, with reference to the relevant literature was identified the, powdery mildew the Erysiphe heraclei DC, Duhuo. The wind leaf spot hazards blades. The lesion intermediate gray-brown, slightly sunken edge with dark brown ridgeline with Physalospora late disease the Ministry with black pellets of points, that pathogen conidia. Pycnidium the foliar Health, scattered or the Poly Health, spherical, flat spherical conidia oval, size (5 -) 7 to 9 (-11) × 2 (-4) ~ 3.5 (-3.9) μm . Long spindle, single cell, colorless. Reference to the relevant literature was identified as celery Phyllosticta Phyllosticta apii Halsted. Major hazard stems of the the windproof stem blight, especially in bolting stems serious, and seriously affect the seed harvest. Occurred at the beginning of bolting the lower part of the stem and bud, after gradually spread to the entire bolting stems, resulting in bolting stems wilting dead stems dense black dots. The stems look unfamiliar pycnidium, scattered or Poly Health, spherical; conidia long spindly, size (6.1-) 6.5 to 10 (-14) × 2.5 (-2.8) to (2.5) 3μm both ends pointed, unicellular, colorless, containing two oil globules. Reference to the relevant literature was identified as fennel Phoma of Phoma foeniculina Saccardo. Of the six diseases, wind bacterial soft rot, bacterial leaf rot, stem blight and abroad have not been reported, therefore, wind is a new record of its corresponding germs host wind leaf spot in Jilin Province is the first time reported. Fungicide screening inhibition zone method and the growth rates of the two kinds of fungal diseases; toxic media law and inhibition zone measured eight kinds of antibiotics wind bacterial soft rot, bacterial leaf rot disease bacteriostatic minimum effective concentration and inhibition zone diameter. The results show that agricultural prop hymexazol, carbendazim wind leaf spot and stem blight inhibitory effect best, preferred pharmaceutical field efficacy trials. Streptomycin not only antibacterial effect is obvious, and the antibacterial lowest effective concentration is lowest, streptomycin for wind bacterial root rot and bacterial leaf rot field efficacy trials preferred pharmacy.

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